Surface engineering of LENS-Ti-6Al-4V to obtain nano- and micro-surface topography for orthopedic application

Revathi A; Mitun Das; Vamsi K Balla; Devika D; Dwaipayan Sen; Geetha Manivasagam

doi:10.1016/j.nano.2019.02.010

Surface engineering of LENS-Ti-6Al-4V to obtain nano- and micro-surface topography for orthopedic application

Nanomedicine. 2019 Jun:18:157-168. doi: 10.1016/j.nano.2019.02.010. Epub 2019 Mar 4.

Authors

Revathi A¹, Mitun Das², Vamsi K Balla², Devika D³, Dwaipayan Sen⁴, Geetha Manivasagam⁵

Affiliations

¹ School of Biosciences and Technology (SBST), VIT University, Vellore, TN, India.
² CSIR-Central Glass and Research Institute, Kolkata, WB, India; School of Mechanical Engineering (SMEC), VIT University, Vellore, TN, India.
³ Department of Mechanical Engineering, Velammal Engineering College, Chennai, TN, India.
⁴ Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), VIT University, Vellore, TN, India.
⁵ Centre for Biomaterials, Cellular and Molecular Theranostics (CBCMT), VIT University, Vellore, TN, India. Electronic address: geethamanivasagam@vit.ac.in.

PMID: 30844575
DOI: 10.1016/j.nano.2019.02.010

Abstract

Two distinct surface topographies consisting of micro- and nano-surface were developed using laser texturing (LT) and anodization process respectively and their effect on the surface-related properties of Ti-6Al-4V fabricated using Laser Engineered Net Shaping (LENS) were determined. The topographies developed using laser texturing (25, 50 and 75% overlap) were examined using 3D profilometer, whereas, Field Emission Scanning Electron Microscopy (FE-SEM) was used to analyze Titania NanoTubes (TNT) formed using anodization. Though all the surface modified specimens exhibited hydrophilic behavior, least contact angle values were observed for the specimen surface modified with TNT. 25LT and 50LT specimens offered about 8 fold higher corrosion resistance than TNT specimens. All the surface modified samples exhibited non-toxicity to blood cells as well as to the mesenchymal stem cells (hMSCs) with a higher rate of proliferation and differentiation hMSCs observed on 75LT specimens and TNT specimen.

Keywords: Biocompatibility; Electron backscatter diffraction (EBSD); Laser engineered net shaping (LENS); Laser texturing; Ti-6Al-4V.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Alkaline Phosphatase / metabolism
Alloys
Dielectric Spectroscopy
Electrochemical Techniques
Hardness
Hemolysis / drug effects
Humans
Lasers*
Male
Materials Testing*
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects
Mesenchymal Stem Cells / enzymology
Mesenchymal Stem Cells / ultrastructure
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Orthopedics / methods*
Surface Properties
Titanium / chemistry*
Titanium / pharmacology

Substances

Alloys
titanium alloy (TiAl6V4)
Titanium
Alkaline Phosphatase