Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells

Elizabeth Guirado; Youbin Zhang; Anne George

doi:10.1007/978-1-4939-9012-2_3

Establishment of Stable Cell Lines from Primary Human Dental Pulp Stem Cells

Methods Mol Biol. 2019:1922:21-27. doi: 10.1007/978-1-4939-9012-2_3.

Authors

Elizabeth Guirado¹, Youbin Zhang¹, Anne George²

Affiliations

¹ Department of Oral Biology, College of Dentistry, The University of Illinois at Chicago, Chicago, IL, USA.
² Department of Oral Biology, College of Dentistry, The University of Illinois at Chicago, Chicago, IL, USA. anneg@uic.edu.

Abstract

This protocol is for the isolation of primary human dental pulp stem cells (DPSCs) from adult extracted molars and for the generation of high-titer lentivirus for in vitro infection of the DPSCs. Stable cell lines of dental pulp stem cells are generated, maintained in culture, and used for subsequent experiments.

Keywords: Gene transfer techniques; Genetic recombination; Genetic transduction; Lentivirus; Somatic stem cells.

MeSH terms

Adult
Cell Culture Techniques / methods*
Cell Line
Cell Separation / methods*
Dental Pulp / cytology*
Gene Transfer Techniques
Humans
Lentivirus
Molar / cytology*
Stem Cells*

Grants and funding

T32 DE018381/DE/NIDCR NIH HHS/United States