Lipidomics differentiation of soft-shelled turtle strains using hydrophilic interaction liquid chromatography and mass spectrometry

Xina Yu; Kang Chen; Shiyan Li; Yang Wang; Qing Shen

doi:10.1016/j.jchromb.2019.02.025

Lipidomics differentiation of soft-shelled turtle strains using hydrophilic interaction liquid chromatography and mass spectrometry

J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Apr 1:1112:11-15. doi: 10.1016/j.jchromb.2019.02.025. Epub 2019 Feb 25.

Authors

Xina Yu¹, Kang Chen¹, Shiyan Li², Yang Wang², Qing Shen³

Affiliations

¹ The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China.
² Aquatic Products Quality Inspection Center of Zhejiang Province, Hangzhou 310012, China.
³ The Joint Key Laboratory of Aquatic Products Processing of Zhejiang Province, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China. Electronic address: sq@zjgsu.edu.cn.

PMID: 30836313
DOI: 10.1016/j.jchromb.2019.02.025

Abstract

Hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) was used as an accurate and sensitivity technique to separate and characterize the phospholipids in three strains of soft-shelled turtles. Under the optimized experimental parameters, the lipidomic profiles were successfully acquired, and a total of 55 phospholipid molecular species were identified. The principal component analysis (PCA) was applied to normalize the relative amounts of the phospholipid molecular species and revealed the differences among three strains of soft-shelled turtle, including Qingxi Huabie (QXH, GS-01-003-2008), Zhexin Wubie (ZXW, GS-02-005-2015), and Zhewu Erhao (ZWE, unregistered). Comparing with other methods, HILIC-MS is rapid, efficient and suitable in determining and visualizing phospholipids. This method also can be used for authenticating different strains of soft-shelled turtles.

Keywords: Hydrophilic interaction liquid chromatography; Lipidomics; Mass spectrometry; Soft-shelled turtle.

MeSH terms

Animals
Chromatography, Liquid / methods*
Computational Biology
Hydrophobic and Hydrophilic Interactions
Linear Models
Mass Spectrometry / methods*
Phospholipids / analysis*
Phospholipids / chemistry
Principal Component Analysis
Reproducibility of Results
Sensitivity and Specificity
Turtles / classification*
Turtles / physiology*

Substances

Phospholipids