Antimicrobial photodynamic activity of toluidine blue encapsulated in mesoporous silica nanoparticles against Pseudomonas aeruginosa and Staphylococcus aureus

Paramanantham Parasuraman; Asha P Antony; Sruthil Lal S B; Alok Sharan; Busi Siddhardha; Kaviyarasu Kasinathan; Needa A Bahkali; Turki M S Dawoud; Asad Syed

doi:10.1080/08927014.2019.1570501

Antimicrobial photodynamic activity of toluidine blue encapsulated in mesoporous silica nanoparticles against Pseudomonas aeruginosa and Staphylococcus aureus

Biofouling. 2019 Jan;35(1):89-103. doi: 10.1080/08927014.2019.1570501. Epub 2019 Mar 5.

Authors

Paramanantham Parasuraman¹, Asha P Antony¹, Sruthil Lal S B², Alok Sharan², Busi Siddhardha¹, Kaviyarasu Kasinathan^{3

4}, Needa A Bahkali⁵, Turki M S Dawoud⁶, Asad Syed⁶

Affiliations

¹ a Department of Microbiology, School of Life Sciences , Pondicherry University , Puducherry , India.
² b Department of Physics, School of Physical, Chemical and Applied Sciences , Pondicherry University , Puducherry , India.
³ c UNESCO-UNISA Africa Chair in Nanoscience's/Nanotechnology Laboratories, College of Graduate Studies , University of South Africa (UNISA) , Ridge , South Africa.
⁴ d Nanosciences African Network (NANOAFNET), Materials Research Group (MRG) , iThemba LABS-National Research Foundation (NRF) , Somerset West , Western Cape Province , South Africa.
⁵ e Biological Sciences Department, Wagner College , Staten Island , NY , USA.
⁶ f Department of Botany and Microbiology, College of Science , King Saud University , Riyadh , Saudi Arabia.

PMID: 30835535
DOI: 10.1080/08927014.2019.1570501

Abstract

In the present study, the antimicrobial and antibiofilm efficacy of toluidine blue (TB) encapsulated in mesoporous silica nanoparticles (MSN) was investigated against Pseudomonas aeruginosa and Staphylococcus aureus treated with antimicrobial photodynamic therapy (aPDT) using a red diode laser 670 nm wavelength, 97.65 J cm^-2 radiant exposure, 5 min). Physico-chemical techniques (UV-visible (UV-vis) absorption, photoluminescence emission, excitation, and FTIR) and high-resolution transmission electron microscopy (HR-TEM) were employed to characterize the conjugate of TB encapsulated in MSN (TB MSN). TB MSN showed maximum antimicrobial activities corresponding to 5.03 and 5.56 log CFU ml^-1 reductions against P. aeruginosa and S. aureus, respectively, whereas samples treated with TB alone showed 2.36 and 2.66 log CFU ml^-1 reductions. Anti-biofilm studies confirmed that TB MSN effectively inhibits biofilm formation and production of extracellular polymeric substances by P. aeruginosa and S. aureus.

Keywords: Antimicrobial photodynamic therapy; anti-biofilm; encapsulation; mesoporous silica nanoparticles; photosensitizer; reactive oxygen species; toluidine blue.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Biofilms / drug effects*
Light
Metal Nanoparticles / chemistry
Nanoparticles
Photochemotherapy / methods*
Pseudomonas aeruginosa / drug effects*
Silicon Dioxide / chemistry*
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
Staphylococcal Infections / drug therapy
Staphylococcus aureus / drug effects*
Tolonium Chloride / pharmacology*

Substances

Anti-Bacterial Agents
Tolonium Chloride
Silicon Dioxide