Novel Hg2+-Selective Signaling Probe Based on Resorufin Thionocarbonate and its μPAD Application

Myung Gil Choi; So Young Park; Ka Young Park; Suk-Kyu Chang

doi:10.1038/s41598-019-40169-6

Novel Hg²⁺-Selective Signaling Probe Based on Resorufin Thionocarbonate and its μPAD Application

Sci Rep. 2019 Mar 4;9(1):3348. doi: 10.1038/s41598-019-40169-6.

Authors

Myung Gil Choi¹, So Young Park¹, Ka Young Park¹, Suk-Kyu Chang²

Affiliations

¹ Department of Chemistry, Chung-Ang University, Seoul, 06974, Republic of Korea.
² Department of Chemistry, Chung-Ang University, Seoul, 06974, Republic of Korea. skchang@cau.ac.kr.

Abstract

In this study, a novel resorufin thionocarbonate-based Hg²⁺-selective signaling probe (RT) for microfluidic paper-based analytical device (μPAD) applications is reported. The designed probe, RT, was readily synthesized by the one-step reaction of resorufin with phenyl thionochloroformate. The RT probe displayed a prominent color change from yellow to pink and a marked turn-on fluorescence signaling behavior exclusively toward the Hg²⁺ ion. The signaling of RT was due to Hg²⁺-induced hydrolysis of the phenyl thionocarbonate moiety to form the parent resorufin dye, which restored its spectroscopic properties. In addition, RT exhibited the Hg²⁺-selective signaling behavior without interference by coexisting environmentally relevant metal ions. The detection limit for Hg²⁺ in simulated wastewater samples was estimated to be 5.8 × 10^-8 M. In particular, an RT-equipped μPAD prepared using a wax printing technique enabled simple and convenient determination of Hg²⁺ ions in simulated wastewater samples, with a detection limit of 5.9 × 10^-6 M.

Publication types

Research Support, Non-U.S. Gov't