Structure characterization and antioxidant activity of fucoidan isolated from Undaria pinnatifida grown in New Zealand

Hui Si Audrey Koh; Jun Lu; Weibiao Zhou

doi:10.1016/j.carbpol.2019.02.040

Structure characterization and antioxidant activity of fucoidan isolated from Undaria pinnatifida grown in New Zealand

Carbohydr Polym. 2019 May 15:212:178-185. doi: 10.1016/j.carbpol.2019.02.040. Epub 2019 Feb 14.

Authors

Hui Si Audrey Koh¹, Jun Lu², Weibiao Zhou³

Affiliations

¹ NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Centre for Life Sciences (CeLS), 28 Medical Drive, Singapore, 117456, Singapore; Food Science and Technology Programme, c/o Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore.
² School of Science, and School of Interprofessional Health Studies, Faculty of Health and Environmental Sciences, Auckland University of Technology, 34 St Paul Street, Auckland, 1142, New Zealand; Institute of Biomedical Technology, Auckland University of Technology, St Paul Street, Auckland, 1142, New Zealand; College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, People's Republic of China; College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, 710062, People's Republic of China.
³ NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Centre for Life Sciences (CeLS), 28 Medical Drive, Singapore, 117456, Singapore; Food Science and Technology Programme, c/o Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore; National University of Singapore (Suzhou) Research Institute, 377 Linquan Street, Suzhou Industrial Park, Jiangsu, 215123, People's Republic of China. Electronic address: chmzwb@nus.edu.sg.

PMID: 30832845
DOI: 10.1016/j.carbpol.2019.02.040

Abstract

Fucoidan from brown seaweed species, Undaria pinnatifida, has numerous bioactive properties such as antioxidant and anticancer activities. The objective of this research was to quantify the chemical composition of fucoidan isolated from U. pinnatifida harvested in New Zealand and to determine its molecular structure and antioxidant capacity. Crude fucoidan (F_crude) and purified fucoidan with molecular weight cut off (MWCO) of 300 kDa (F₃₀₀) were compared against a fucoidan standard (F_Std). F_Std contained significantly higher sulfate content as compared to F_crude and F₃₀₀. Based on FTIR and 2D HSQC analysis, it was proposed that the purified fucoidan was composed of an alternating fucose-galactose backbone linked together via 1,3 glycosidic bonds with sulfation at C2 and C4 positions. Fucoidan samples from New Zealand exhibited secondary antioxidant capacity close to that of butylated hydroxyanisole (BHA), with low-molecular-weight fucoidan had significantly higher capacity than that of BHA.

Keywords: Antioxidant; Fourier transform infra-red (FTIR); Fucoidan; Nuclear magnetic resonance (NMR); Undaria pinnatifida.

MeSH terms

Antioxidants / chemistry*
Antioxidants / isolation & purification*
Magnetic Resonance Spectroscopy / methods
Molecular Structure
New Zealand
Polysaccharides / chemistry*
Polysaccharides / isolation & purification*
Spectroscopy, Fourier Transform Infrared / methods
Undaria*

Substances

Antioxidants
Polysaccharides
fucoidan