Comparative investigation on metabolite changes in 'wu mi' production by Vaccinium bracteatum Thunb. leaves based on multivariate data analysis using UPLC-QToF-MS

Mingcong Fan; Yihui Fan; Zhiming Rao; Yan Li; Haifeng Qian; Hui Zhang; Gangcheng Wu; Xiguang Qi; Li Wang

doi:10.1016/j.foodchem.2019.01.144

Comparative investigation on metabolite changes in 'wu mi' production by Vaccinium bracteatum Thunb. leaves based on multivariate data analysis using UPLC-QToF-MS

Food Chem. 2019 Jul 15:286:146-153. doi: 10.1016/j.foodchem.2019.01.144. Epub 2019 Jan 31.

Authors

Mingcong Fan¹, Yihui Fan², Zhiming Rao³, Yan Li⁴, Haifeng Qian⁴, Hui Zhang⁴, Gangcheng Wu⁴, Xiguang Qi⁴, Li Wang⁵

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
² Department of Immunology, School of Medicine, Nantong University, 19 Qixiu Road, Nantong 226001, China.
³ School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China.
⁵ State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. Electronic address: wl0519@163.com.

PMID: 30827588
DOI: 10.1016/j.foodchem.2019.01.144

Abstract

Vaccinium bracteatum Thunb. leaves (VBTL) are used to juicing and dye rice to produce 'Wu mi', which displays a deep blue color. However, little information is known about the formation mechanism of the pigments. In this research, non-targeted metabolic profiling of VBTL and VBTL juice samples at different growth stages was studied for searching the pigment precursors through UPLC-QToF-MS and multivariate data analysis. The results showed the L^* and b^* values of 'Wu mi' produced by spring leaves (stages of 2WAB, 4WAB and 6WAB) were significantly lower than other growth stages. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) of the VBTL and VBTL juice samples showed distinct classifications. Further variable importance in projection (VIP) plot in PLS-DA model demonstrated the discriminatory potential biomarkers between VBTL and VBTL juice samples. Some of the identified biomarkers were tentatively identified as the precursor compounds of the iridoid-derived pigments.

Keywords: Color; Multivariate data analysis; Potential metabolites; Vaccinium bracteatum Thunb. leaves (VBTL); Wu mi.

Publication types

Comparative Study

MeSH terms

Chromatography, High Pressure Liquid / methods
Discriminant Analysis
Fruit and Vegetable Juices / analysis
Least-Squares Analysis
Mass Spectrometry / methods*
Mass Spectrometry / statistics & numerical data
Metabolomics / methods*
Metabolomics / statistics & numerical data
Multivariate Analysis
Oryza
Plant Leaves / metabolism*
Vaccinium myrtillus / metabolism*