Toll-like receptors (TLRs) play crucial roles in the host immune system, including recognizing invading pathogenic microbes and triggering immune reactions. Toll-like receptor 14 (TLR14) has been identified in several fish species, but its function requires further study. In this study, TLR14 (designed as ToTLR14) from golden pompano (Trachinotus ovatus), was characterized and investigated its expression responses to three types of pathogen-associated molecular patterns. The full-length ToTLR14 cDNA was 3191 bp, and the deduced protein consisted of 876 amino acids. The ToTLR14 protein included 5 leucine rich repeat (LRR) domains, a C-terminal LRR domain in the extracellular region, a transmembrane domain and a Toll/interleukin (IL)-1 receptor (TIR) domain in the cytoplasmic region, which fits with the typical TLR domain architecture. The genomic organization of ToTLR14 was also identified and consisted of four introns and five exons. The predicted promoter region of ToTLR14 contained several putative transcription factor binding sites. Phylogenetic analysis showed that ToTLR14 was clustered into the TLR1 subfamily clade. Quantitative real-time (qRT-PCR) analysis indicated that ToTLR14 were ubiquitously expressed in all examined tissues, with higher mRNA levels observed in the skin, kidney and intestine, while the lowest level was detected in the stomach. After injection with polyinosinic:polycytidylic acid [poly(I:C)], flagellin or lipopolysaccharides (LPS), the expression level of ToTLR14 mRNA were significantly upregulated in various tissues of golden pompano. These results indicate that ToTLR14 may play an important role in systemic as well as mucosal defence after viral and bacterial stimulation.
Keywords: Expression regulation; Immune response; Toll-like receptor 14; Trachinotus ovatus.
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