Methods for Isolation and Characterization of Sulfated Glycosaminoglycans from Marine Invertebrates

Methods Mol Biol. 2019:1952:55-70. doi: 10.1007/978-1-4939-9133-4_5.

Abstract

Marine invertebrates produce different kinds of sulfated polysaccharides. These glycans play essential roles in several biological processes and the study of these molecules is promising in a variety of fields. In the following sections, we describe the materials and methods used for the extraction, purification, and characterization of marine invertebrate-derived glycosaminoglycans.

Keywords: Marine invertebrates; Nuclear magnetic resonance; Polysaccharide purification; Sulfated glycosaminoglycans; Sulfated polysaccharide characterization.

MeSH terms

  • Animals
  • Aquatic Organisms / chemistry
  • Chemical Precipitation
  • Chromatography, Ion Exchange / methods
  • Dissection / methods
  • Electrophoresis, Agar Gel / methods
  • Glycosaminoglycans / chemistry*
  • Glycosaminoglycans / isolation & purification
  • Invertebrates / chemistry
  • Magnetic Resonance Spectroscopy / methods
  • Proteolysis
  • Sulfates / chemistry*
  • Sulfates / isolation & purification

Substances

  • Glycosaminoglycans
  • Sulfates