Biosensor experiments on investigation of interaction between prostacyclin synthase (PGIS) and different proteins of the cytochrome P450 monooxygenase systems were perfomed. Interaction of PGIS with microsomal (CYP21A2, CYP2E1) and mitochondrial (CYP27A1, CYP11B1, CYP11B2, CYP11A1) cytochrome P450s was detected. Kinetic and equilibrium parameters of protein complexes formation were determined. Data obtained suggest an essential role of these hemoproteins interaction in regulation of prostacyclin and thromboxane A2 biosynthesis.
Vypolneny biosensornye éksperimenty po issledovaniiu vzaimodeĭstviia prostatsiklinsintazy (PGIS) s razlichnymi belkami tsitokhrom P450-zavisimykh monooksigenaznykh sistem. Obnaruzheno vzaimodeĭstvie PGIS s mikrosomal'nymi (CYP21A2, CYP2E1) i mitokhondrial'nymi (CYP27A1, CYP11B1, CYP11B2, CYP11A1) tsitokhromami P450. Opredeleny kineticheskie i ravnovesnye parametry formirovaniia belkovykh kompleksov. Poluchennye rezul'taty pozvoliaiut predpolozhit', chto vzaimodeĭstvie dannykh gemoproteinov mozhet igrat' vazhnuiu rol' v reguliatsii biosinteza kak prostatsiklina, tak i ego fiziologicheskogo protivovesa tromboksana A2.
Keywords: cytochrome P450; optical biosensor Biacore; prostacyclin synthase (PGIS CYP8A1); surface plasmon resonance (SPR).