Organic lithium salts containing anionic components (succinate, fumarate, pyruvate and antioxidant ascorbate) were tested for protection of blood plasma proteins and lipids against ethanol-induced oxidation in vitro. We used normothymic lithium carbonate and well-known antioxidant dipeptide carnosine (b-alanyl-L-histidine) as the reference drugs. The oxidized proteins and lipids were determined by the level of carbonylated proteins (CP) and TBA-reactive products (TBA-RP), respectively. In alcoholic patients the level of oxidized proteins and lipids was higher than in healthy persons. Incubation of blood with ethanol resulted in an increase in oxidized proteins and lipids in blood plasma of healthy persons but had no influence on the level of CP and TBA-RP in blood plasma of alcoholic patients. Lithium carbonate, lithium ascorbate, and lithium succinate exhibited protective action against ethanol-induced oxidation of biomolecules of blood plasma of healthy people. These effects were comparable with carnosine action. The studied compounds had no effect on the level of CP and TBA-RP of blood plasma of alcoholic patients.
Issledovali zashchitu belkov i lipidov plazmy krovi ot okisleniia étanolom pri ispol'zovanii organicheskikh soleĭ litiia, soderzhashchikh v svoem sostave anionnye komponenty (suktsinat, fumarat, piruvat), a takzhe antioksidant askorbat. V kachestve preparatov sravneniia ispol'zovali normotimik karbonat litiia i izvestnyĭ antioksidant dipeptid karnozin (b-alanil-L-gistidin). Okislennye belki opredeliali po urovniu karbonilirovannykh belkov (KB), okislitel'nuiu modifikatsiiu lipidov – po TBK-reaktivnym produktam (TBK-RP). U bol'nykh alkogolizmom uroven' okislennykh belkov i lipidov povyshen po sravneniiu so zdorovymi litsami. Inkubatsiia krovi s étanolom privodit k uvelicheniiu okislennykh belkov i lipidov v plazme krovi zdorovykh lits, no ne vliiaet na uroven' okislennykh biomolekul v plazme krovi bol'nykh alkogolizmom. Zashchitnoe deĭstvie ot étanol-indutsirovannogo okisleniia biomolekul plazmy krovi zdorovykh liudeĭ, sopostavimoe po éffektivnosti s karnozinom, okazyvali karbonat litiia, askorbat litiia i suktsinat litiia. Vliianiia issleduemykh soedineniĭ na uroven' KB i TBK-RP plazmy krovi bol'nykh alkogolizmom ne obnaruzheno.
Keywords: alcoholism; antioxidants; biomolecules of plasma; ethanol-induced oxidation; organic lithium salts.