Detection of phytochemical compounds and pigments in seeds and shoots of Cuscuta campestris parasitizing on eggplant

Physiol Mol Biol Plants. 2019 Jan;25(1):253-261. doi: 10.1007/s12298-018-0630-4. Epub 2018 Dec 14.

Abstract

This study aimed to detect the chemical compounds contained in Cuscuta campestris seeds and shoot parts using the GC-MS following methanol extraction method, and also determining protein, total phenolic, total soluble carbohydrates, plant hormones and pigments for both seeds and shoot (flower and filament). The GC-MS analysis results showed six compounds are common between seed and shoot and the presence of different compounds in different plant parts including 24 compounds in seeds and 9 compounds in the shoot parts. The content of protein and carbohydrate in shoot (1.095% and 22.640 mg g-1 respectively) were higher than in seeds and the total phenols content in seeds (11.094 mg g-1) was greatest than in shoot. In addition, there were differences between seeds and filaments parts in terms of concentration of pigments and free plant hormones, filaments were superior in total chlorophyll and carotene content (5.37 mg 100 g-1 and 13.26 mg 100 g-1 respectively). In seeds, ABA content was very high reaching 93.38 µM compared to other hormones, which had an effect on the seed dormancy of C. campestris.

Keywords: C. campestris; Eggplant; Filaments; GC–MS; Seeds; Shoot.