Phage displayed mimotope peptide-based immunosensor for green and ultrasensitive detection of mycotoxin deoxynivalenol

Jiaxiang Yan; Qiang Shi; Kaihao You; Yanping Li; Qinghua He

doi:10.1016/j.jpba.2019.01.051

Phage displayed mimotope peptide-based immunosensor for green and ultrasensitive detection of mycotoxin deoxynivalenol

J Pharm Biomed Anal. 2019 May 10:168:94-101. doi: 10.1016/j.jpba.2019.01.051. Epub 2019 Jan 31.

Authors

Jiaxiang Yan¹, Qiang Shi², Kaihao You², Yanping Li³, Qinghua He⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, No. 999 Xuefu Avenue, Nanchang 330031, China.
² School of Food Science and Technology, Nanchang University, No. 999 Xuefu Avenue, Nanchang 330031, China.
³ State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, No. 999 Xuefu Avenue, Nanchang 330031, China.
⁴ State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, No. 999 Xuefu Avenue, Nanchang 330031, China. Electronic address: heqinghua@ncu.edu.cn.

PMID: 30802751
DOI: 10.1016/j.jpba.2019.01.051

Abstract

Immunoassays play an important role in the monitoring and detection of mycotoxins in food. However, mycotoxin conjugates in the immunoassay system synthesized with toxic mycotoxin as raw material has seriously harmed the environment and human health during and after the performance. In this work, using mycotoxin deoxynivalenol (DON) as a model happen, a selected DON mimotope (phage-displayed peptide) that specifically binds to anti-DON antibody was served as a mimetic competing antigen in electrochemical immunosensor for ultrasensitive and green detection of DON. Under the optimized conditions, the mimotope peptide-based electrochemical immunosensor showed a dynamic range of 0.1-10,000 pg/ml and the limit detection (LOD) of 0.07 pg/ml. In addition, the accuracy and reproducibility of the established electrochemical immunosensor were evaluated by testing cross-reactivity and spiking DON negative samples. The result showed that mimotope peptide-based immunosensor has negligible cross-reactivity with other mycotoxins, and the average recovery of corn and wheat sample was 90.4%-118% and 101.3%-111%, respectively. Furthermore, the novel concept might provide potential applications to a general method for the immunosensor of various toxic small molecules.

Keywords: Deoxynivalenol; Electrochemical immunosensor; Mimotope; Phage displayed peptide.

MeSH terms

Cell Surface Display Techniques / methods*
Electrochemical Techniques / methods
Green Chemistry Technology / methods
Immunoassay / methods*
Limit of Detection
Mycotoxins / analysis*
Peptides / chemistry
Reproducibility of Results
Trichothecenes / analysis*

Substances

Mycotoxins
Peptides
Trichothecenes
deoxynivalenol