Draft genome sequences of three clinical isolates of teicoplanin-resistant Staphylococcus epidermidis from patients without prior exposure to glycopeptide antibiotics

Vladimir Vimberg; Leona Zieglerova; Jan Závora; Lenka Šemberová; Jana Prásilová; Václava Adámková; Gabriela Balikova Novotna

doi:10.1016/j.jgar.2019.02.005

Draft genome sequences of three clinical isolates of teicoplanin-resistant Staphylococcus epidermidis from patients without prior exposure to glycopeptide antibiotics

J Glob Antimicrob Resist. 2019 Mar:16:251-253. doi: 10.1016/j.jgar.2019.02.005. Epub 2019 Feb 20.

Authors

Vladimir Vimberg¹, Leona Zieglerova², Jan Závora³, Lenka Šemberová³, Jana Prásilová², Václava Adámková⁴, Gabriela Balikova Novotna²

Affiliations

¹ Laboratory for Biology of Secondary Metabolism, Institute of Microbiology of the Academy of Sciences of the Czech Republic, BIOCEV, Průmyslová 595, 252 50 Vestec, Czech Republic. Electronic address: vladimir.vimberg@biomed.cas.cz.
² Laboratory for Biology of Secondary Metabolism, Institute of Microbiology of the Academy of Sciences of the Czech Republic, BIOCEV, Průmyslová 595, 252 50 Vestec, Czech Republic.
³ Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, Prague, Czech Republic.
⁴ Clinical Microbiology and ATB Centre, Institute of Medical Biochemistry and Laboratory Diagnostics, General University Hospital, Prague, Czech Republic; Department of Medical Microbiology, Medical Faculty of Palacký University, Olomouc, Czech Republic.

PMID: 30797086
DOI: 10.1016/j.jgar.2019.02.005

Abstract

Objectives: The aim of this study was to analyse the DNA sequences of three teicoplanin-resistant Staphylococcus epidermidis isolates collected from patients not previously treated with glycopeptide antibiotics.

Methods: The minimum inhibitory concentrations (MICs) of 12 antibiotics, including teicoplanin and vancomycin, were determined by the broth microdilution method. Genomic DNA was isolated, was sequenced by HiSeqX paired-end sequencing and was assembled into draft genome sequences using MyPro pipeline.

Results: Analysis of the draft genome sequences demonstrated that the teicoplanin-resistant S. epidermidis isolates belonged to multilocus sequence typing (MLST) sequence types ST5 and ST87 and encoded multiple antimicrobial resistance genes, including the methicillin resistance gene mecA.

Conclusions: This report highlights the risk of dissemination of S. epidermidis strains resistant to a wide range of clinically important antibiotics.

Keywords: Staphylococcus epidermidis; Teicoplanin resistance; Whole-genome sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Typing Techniques
Drug Resistance, Bacterial*
Genome, Bacterial*
Humans
Microbial Sensitivity Tests
Multilocus Sequence Typing
Staphylococcal Infections / microbiology*
Staphylococcus epidermidis / classification*
Staphylococcus epidermidis / drug effects
Teicoplanin / pharmacology*
Whole Genome Sequencing

Substances

Anti-Bacterial Agents
Teicoplanin