Circular RNA expression in exosomes derived from breast cancer cells and patients

Epigenomics. 2019 Feb;11(4):411-421. doi: 10.2217/epi-2018-0111. Epub 2019 Feb 20.

Abstract

Aim: We aimed to explore the roles of circular RNAs (circRNAs) in breast cancer (BCa).

Materials & methods: RNA was extracted from exosomes and BCa cells and analyzed using the RNA sequencing technique or microarray.

Results: Compared with controls, 1147 and 1195 circRNAs were dysregulated in exosomes from metastatic and localized BCa patients, respectively. A total of 480 dysregulated circRNAs were found in metastatic patients compared with localized patients, and these dysregulated circRNAs were enriched in eight pathways. Compared with MCF-7 cells and their exosomes, there were 5842 and 1137 dysregulated circRNAs in MDA-MB-231 cells and exosomes, respectively, and 5 circRNAs were confirmed using real-time quantitative PCR.

Conclusion: We identified a number of dysregulated circRNAs in exosomes from BCa cells and patients.

Keywords: breast cancer; breast carcinoma; carcinogenesis; circular RNA; exosomes; extracellular vesicles; invasiveness; metastasis; miRNA; pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor*
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • Cell Line, Tumor
  • Computational Biology / methods
  • Exosomes / metabolism*
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Gene Regulatory Networks
  • Humans
  • RNA, Circular*

Substances

  • Biomarkers, Tumor
  • RNA, Circular