The effect of ultraviolet-C and precursor feeding on stilbene biosynthesis in spruce Picea jezoensis

K V Kiselev; V P Grigorchuk; Z V Ogneva; A R Suprun; A S Dubrovina

doi:10.1016/j.jplph.2019.02.002

The effect of ultraviolet-C and precursor feeding on stilbene biosynthesis in spruce Picea jezoensis

J Plant Physiol. 2019 Mar-Apr:234-235:133-137. doi: 10.1016/j.jplph.2019.02.002. Epub 2019 Feb 14.

Authors

K V Kiselev¹, V P Grigorchuk², Z V Ogneva³, A R Suprun³, A S Dubrovina²

Affiliations

¹ Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia; Department of Biodiversity, The School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia. Electronic address: kiselev@biosoil.ru.
² Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia.
³ Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia; Department of Biodiversity, The School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia.

PMID: 30784851
DOI: 10.1016/j.jplph.2019.02.002

Abstract

This study examines the effects of p-coumaric (CoA) and caffeic (CaA) acids on stilbene biosynthesis in one-year-old needles of Picea jezoensis (Lindl. et Gord.) Fisch ex Carr. under control conditions and after ultraviolet (UV-C) irradiation. HPLC analysis revealed that while UV-C irradiation slightly affected the total stilbene content, CoA and CaA feeding increased the total content of stilbenes by 1.2-1.3-fold. UV-C treatment combined with CoA-feeding of the P. jezoensis cuttings exerted the most pronounced stimulatory effect on stilbene accumulation leading to the total stilbene content of 9.18 mg/g of dry weight or DW. This increase correlated with the elevated transcription of the stilbene synthase PjSTS1a and PjSTS1b genes. UV-C treatment in combination with CaA feeding of P. jezoensis did not considerably influence stilbene content. These results revealed a positive effect of UV-C radiation and phenolic precursors on the content of stilbenes in spruce needles.

Keywords: Astringin; Isorhapontin; Piceatannol; Piceid; Resveratrol; STS expression.

MeSH terms

Caffeic Acids
Coumaric Acids
Gene Expression
Picea / genetics
Picea / metabolism*
Picea / radiation effects
Stilbenes / metabolism*
Ultraviolet Rays

Substances

Caffeic Acids
Coumaric Acids
Stilbenes
caffeic acid