AAV-Mediated Gene Delivery to the Lung

Laura P van Lieshout; Jakob M Domm; Sarah K Wootton

doi:10.1007/978-1-4939-9139-6_21

AAV-Mediated Gene Delivery to the Lung

Methods Mol Biol. 2019:1950:361-372. doi: 10.1007/978-1-4939-9139-6_21.

Authors

Laura P van Lieshout¹, Jakob M Domm¹, Sarah K Wootton²

Affiliations

¹ Department of Pathobiology, University of Guelph, Guelph, ON, Canada.
² Department of Pathobiology, University of Guelph, Guelph, ON, Canada. kwootton@uoguelph.ca.

PMID: 30783985
DOI: 10.1007/978-1-4939-9139-6_21

Abstract

Adeno-associated virus (AAV) vectors are an efficient method of gene delivery to various tissues including the lung. Mouse models are often used as a preliminary preclinical model in order to advance AAV lung gene therapy vectors. In this chapter we describe an AAV purification protocol using heparin affinity chromatography as well as an intranasal and intratracheal method of delivering AAV vectors to the lungs of mice.

Keywords: AAV; AAV purification; AAV6.2FF; Adeno-associated virus; Heparin column chromatography; Intranasal instillation; Lung delivery; Lung gene therapy; Lung gene transfer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Intranasal
Animals
Cell Line
Chromatography
Dependovirus / genetics*
Gene Transfer Techniques*
Genetic Vectors / administration & dosage
Genetic Vectors / genetics*
Genetic Vectors / isolation & purification
Humans
Injection, Intratympanic
Lung / metabolism*
Mice
Transduction, Genetic