In this work, we have synthesized a novel graphene oxide/trans-1,4-polyisoprene (GO/TPI) shape memory polymer (SMP) nanocomposite by adding GO (0.0-1.5 phr) to bulk TPI polymer to enhance its mechanical properties. We have studied and examined its mechanical, thermal and thermomechanical properties through systematic tests from microscale to macroscale. The shape memory properties and the effect of the GO content on the thermal and mechanical properties of the manufactured SMP nanocomposites were studied by differential scanning calorimetry, dynamic mechanical analysis, thermogravimetric analysis, thermal conductivity, and static tensile and mechanical as well as thermomechanical cyclic loading tests. The experimental results indicate that the SMP nanocomposite with 0.9 phr GO has superior thermal and mechanical properties compared to all other cases, and good shape memory was obtained in the GO/TPI nanocomposites.