Many methods exist to study oncogenic signaling in lymphoma cells, each with certain strengths and weaknesses. The proximity ligation assay (PLA) is a technique used to examine protein-protein interactions in situ. PLA offers many advantages over traditional biochemical approaches to signaling in that it can detect both transient interactions, which are hard to capture otherwise, as well as protein interactions within large supramolecular signaling platforms that are often intractable to traditional biochemical methodologies. In addition, PLA can be adapted for use in lymphoma biopsy samples to translate findings from the lab. Implementation of PLA can complement many types of lymphoma research, and the methods presented herein can be adapted to a number of experimental settings.
Keywords: Biochemistry; Clinical research; Lymphoma; Protein-protein interactions; Proximity ligation assay.