The separation and purification techniques of chelates can improve the accuracy of detecting results of the chelation rate. As a quantitative indicator of metal ion chelates, the chelation rate can not only reflect the completion of chelation but also determine the amount of metal ions in different forms. The determination of chelation rate can help to determine the suitable chelating reaction conditions, make theoretical basis for the fertilizer efficiency, analyze the stability of chelating fertilizers and study the action mechanism of trace elements. In our study, the methods of separation free metal ions from mixture were reviewed first, including gel filtration chromatography, organic solvent precipitation, ion exchange chromatography, membrane separation and high performance liquid chromatography. Then, the qualitative analysis methods of chelates were introduced briefly, including chemical identification, infrared spectroscopy, ultraviolet spectroscopy. A detailed overview of the quantitative determination methods of chelates were also shown, such as ethylenediaminetetraacetic acid titration, chemical titration, atomic absorption spectrometry, inductively coupled plasma atomic emission spectrometry, inductively coupled plasma mass spectrometry, spectrophotometric, chemical modified electrode. In addition, the merits and demerits of chelated rate determination methods of various determination methods were analyzed, and summarized the applicability of various methods, which provided a theoretical basis for optimizing chelating process, characterizing the structure of chelates and analyzing the mechanism of chelating fertilizer. The current methods of measuring chelation rate were also summarized and prospected.
Keywords: Chelates; chelation rate; qualitative analysis; separation and purification.