Magnetic immobilization of a quorum sensing signal hydrolase, AiiA

Microbiologyopen. 2019 Aug;8(8):e00797. doi: 10.1002/mbo3.797. Epub 2019 Feb 14.

Abstract

Magnetic immobilization of quorum sensing (QS) signal hydrolases provides a convenient solution for quenching QS process that is essential for bacterial biofilm formation and antimicrobial resistance. In the present study, a QS signal hydrolase, AiiA, was fused with a magnetic protein, MagR, and expressed in Escherichia coli. Magnetic immobilization of AiiA was achieved on Fe3 O4 -SiO2 iron beads and was confirmed via SDS-PAGE, zeta potential measurement, FTIR spectrometry, and SEM analysis. The magnetic immobilized AiiA exhibited activity in degrading the quorum sensing signal, C6-HSL. This study opens a new avenue to actively immobilize enzymes via magnetic interaction and quench quorum sensing.

Keywords: AiiA; MagR; hydrolase; magnetic immobilization; quorum sensing signal.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyl-Butyrolactones / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Enzymes, Immobilized / genetics
  • Enzymes, Immobilized / metabolism*
  • Escherichia coli / genetics
  • Gene Expression
  • Iron-Sulfur Proteins / genetics
  • Iron-Sulfur Proteins / metabolism*
  • Magnetics*
  • Metalloendopeptidases / genetics
  • Metalloendopeptidases / metabolism*
  • Microspheres
  • Protein Binding
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism*

Substances

  • Acyl-Butyrolactones
  • Bacterial Proteins
  • Drosophila Proteins
  • Enzymes, Immobilized
  • Iron-Sulfur Proteins
  • MagR protein, Drosophila
  • Recombinant Fusion Proteins
  • AiiA protein, Bacillus
  • Metalloendopeptidases