Multiple sgRNAs facilitate base editing-mediated i-stop to induce complete and precise gene disruption

Affiliations

• ¹ Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing, 100094, China.
• ² School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
• ³ Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University, Suzhou, 215123, China.
• ⁴ Key Laboratory of MEMS of Ministry of Education, Southeast University, Nanjing, 210096, China.
• ⁵ Cambridge-Suda Genomic Resource Center and Jiangsu Key Laboratory of Neuropsychiatric Diseases Research, Soochow University, Suzhou, 215123, China. pandejing@suda.edu.cn.
• ⁶ Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing, 100094, China. lianzhx@cau.edu.cn.