Inhibition of pathogenic bacterial biofilms on PDMS based implants by L. acidophilus derived biosurfactant

Surekha K Satpute; Nishigandha S Mone; Parijat Das; Ibrahim M Banat; Arun G Banpurkar

doi:10.1186/s12866-019-1412-z

Inhibition of pathogenic bacterial biofilms on PDMS based implants by L. acidophilus derived biosurfactant

BMC Microbiol. 2019 Feb 13;19(1):39. doi: 10.1186/s12866-019-1412-z.

Authors

Surekha K Satpute¹, Nishigandha S Mone², Parijat Das^{2

3}, Ibrahim M Banat⁴, Arun G Banpurkar⁵

Affiliations

¹ Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India. drsurekhasatpute@gmail.com.
² Department of Microbiology, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India.
³ Present Address: Protein Crystallography lab (603), Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, Maharashtra, 400076, India.
⁴ School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, N., Ireland, UK.
⁵ Department of Physics, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India. arunbanpurkar@gmail.com.

Abstract

Background: Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Therefore, the objective of our research is to investigate the in-vitro conditions for the prospective of medically valuable biosurfactants (BSs) derived from Lactobacillus spp. Biosurfactant (BS) obtained from Lactobacillus spp. exhibit antibiofilm and antiadhesive activity against broad range of microbes. In the present study we investigated the production, purification and properties of key components of the cell-associated-biosurfactant (CABS) from Lactobacillus acidophilus NCIM 2903.

Results: Extracted, purified, freeze-dried CABS shows reduction in surface tension (SFT) of phosphate buffer saline (PBS @pH 7.0) from 71 to 26 mN/m and had a critical micelle concentration (CMC) of 23.6 mg/mL. The CABS showed reduction in interfacial tension (IFT) against various hydrocarbons and had effective spreading capability as reflected through the decrease in contact angle (CA) on different surfaces (polydimethylsiloxane - PDMS, Teflon tape, glass surface, polystyrene film and OHP sheet). The anionic nature of CABS displayed stability at different pH and temperatures and formed stable emulsions. Thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FTIR) revealed CABS as glycolipoprotein type. The Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed presence of multiple bands in a molecular range of 14.4 to 60 kDa, with prominent bands of 45 kDa. The CABS has significant antiadhesion and antibiofilm activity against tested bacterial strains.

Conclusion: The current challenging situation is to develop methods or search for the molecules that will prevent the formations of biofilm on medical bioimplants of PDMS based materials. These findings are supportive for the use of Lactobacilli derived BS as potential antiadhesive agent on various surfaces of biomedical devices.

Keywords: Adhesion; Biofilm; Biosurfactant; Lactobacillus; Medical implants, PDMS, protein, surface tension.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anions
Bacteria / drug effects*
Bacteria / pathogenicity
Bacterial Adhesion / drug effects*
Biofilms / drug effects*
Glass
Lactobacillus / chemistry*
Polystyrenes
Polytetrafluoroethylene
Spectroscopy, Fourier Transform Infrared
Surface Properties
Surface-Active Agents / chemistry
Surface-Active Agents / isolation & purification
Surface-Active Agents / pharmacology*

Substances

Anions
Polystyrenes
Surface-Active Agents
Polytetrafluoroethylene