1H, 13C, and 15N resonance assignments of the cytokine interleukin-36β isoform-2

Biomol NMR Assign. 2019 Apr;13(1):155-161. doi: 10.1007/s12104-018-09869-4. Epub 2019 Feb 13.

Abstract

Interleukins are cytokines performing central tasks in the human immune system. Interleukin-36β (IL-36β) is a member of the interleukin-1 superfamily as are its homologues IL-36α and IL-36γ. All of them interact with a common receptor composed of IL-36R and IL-1R/acP. IL-36 cytokines can activate IL-36R to proliferation of CD4 + lymphocytes or stimulate M2 macrophages as potently as IL-1β. Within our efforts to study the structure-function relationship of the three interleukins IL-36α, IL-36β and IL-36γ by heteronuclear multidimensional NMR, we here report the 1H, 13C, and 15N resonance assignments for the backbone and side chain nuclei of cytokine interleukin-36β isoform-2.

Keywords: Cytokines; Heteronuclear NMR; Interleukin-1 superfamily; Interleukin-36β; Resonance assignments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carbon Isotopes
  • Deuterium Exchange Measurement
  • Humans
  • Hydrogen Bonding
  • Interleukin-1 / chemistry*
  • Nitrogen Radioisotopes
  • Nuclear Magnetic Resonance, Biomolecular*
  • Protein Isoforms / chemistry
  • Protons
  • Temperature

Substances

  • Carbon Isotopes
  • IL36B protein, human
  • Interleukin-1
  • Nitrogen Radioisotopes
  • Nitrogen-13
  • Protein Isoforms
  • Protons
  • Carbon-13