Cystic echinococcosis and toxoplasmosis are two human parasitic diseases. Butchers and slaughterhouse workers are in close contact with body fluids and tissues of ruminants. To investigate the prevalence of Toxoplasma and Echinococcus IgG antibodies in slaughterhouse workers in Northeast of Iran, Mashhad, 2016. This cross-sectional study was performed on all personnel working at the largest industrial slaughterhouse of Khorasan Province. Serum samples were taken and kept frozen until used. IgG against Echinococcus and Toxoplasma were quantified using commercial ELISA kits. A questionnaire addressing possible risk factors of infection acquisition was filled by participants. Out of 91 male participants, 58.2% were positive for toxoplasmosis, 5.5% were positive for cystic echinococcosis and 3.3% were positive for both. Except using gloves, and gown and boots, other personal protective equipment (PPE) were not completely used by all personnel; mask 38%, tool disinfectants: 12%, face and hand disinfection: 14.3%. There was a high risk of Toxoplasma in slaughterhouse workers, however, such finding was not observed in Echinococcus parasite. Importance of PPE and tool disinfectants to reduce the risk of zoonotic infections in the workplace should be emphasised.