Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics

Roslyn M Bill; Annegret Ulke-Lemée; Stephanie P Cartwright; Rena Far; Jay Kim; Justin A MacDonald

doi:10.1007/978-1-4939-9024-5_8

Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics

Methods Mol Biol. 2019:1923:187-209. doi: 10.1007/978-1-4939-9024-5_8.

Authors

Roslyn M Bill¹, Annegret Ulke-Lemée², Stephanie P Cartwright¹, Rena Far², Jay Kim³, Justin A MacDonald⁴

Affiliations

¹ School of Life and Health Sciences, Aston University, Birmingham, UK.
² Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
³ Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada.
⁴ Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. jmacdo@ucalgary.ca.

PMID: 30737741
DOI: 10.1007/978-1-4939-9024-5_8

Abstract

New mass spectrometry approaches enable antibody-independent tracking of protein production. Herein, we outline an antibody-independent mass spectrometry method for tracking recombinant protein production in the methylotrophic yeast Pichia pastoris system.

Keywords: DAPK3; MRM-MS; Mass spectrometry; Multiple reaction monitoring; Recombinant protein production; Yeast; ZIPK.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Mass Spectrometry / methods*
Pichia / genetics
Pichia / metabolism*
Proteomics / methods*
Recombinant Proteins / genetics
Recombinant Proteins / metabolism*

Substances

Recombinant Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding