Techniques for the Study of Apoptosis in Bone

Val Mann; Brendon Noble

doi:10.1007/978-1-4939-8997-3_26

Techniques for the Study of Apoptosis in Bone

Methods Mol Biol. 2019:1914:451-465. doi: 10.1007/978-1-4939-8997-3_26.

Authors

Val Mann¹, Brendon Noble²

Affiliations

¹ Faculty of Medicine and Dentistry, University of Plymouth, Plymouth, UK.
² School of Life Sciences, University of Westminster, London, UK.

PMID: 30729482
DOI: 10.1007/978-1-4939-8997-3_26

Abstract

Osteocyte apoptosis has been associated with a number of clinical conditions in bone and with the targeted turnover of specific skeletal areas. There has been great interest in the identification of the mechanisms by which apoptosis is regulated in bone and in the biological role that this process plays in bone metabolism and associated bone disease or loss of structural integrity. Here we describe several methods for the detection of apoptosis in bone sections and in bone cell cultures.

Keywords: Apoptosis; Bone; Caspase; Nick translation; Osteocyte.

MeSH terms

Animals
Apoptosis / physiology*
Bone and Bones / cytology
Bone and Bones / physiology*
Caspases / analysis
Caspases / metabolism
Cell Culture Techniques / instrumentation
Cell Culture Techniques / methods
Cell Survival / physiology
Cells, Cultured
Humans
In Situ Nick-End Labeling / instrumentation
In Situ Nick-End Labeling / methods
Indoles / chemistry
L-Lactate Dehydrogenase / chemistry
Osteocytes / metabolism
Osteocytes / physiology*
Staining and Labeling / instrumentation
Staining and Labeling / methods
Tolonium Chloride / chemistry

Substances

Indoles
Tolonium Chloride
DAPI
L-Lactate Dehydrogenase
Caspases