Genome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3

Yuriy Kuzhyk; Maria Lopatniuk; Andriy Luzhetskyy; Victor Fedorenko; Bohdan Ostash

doi:10.1007/s12088-018-0761-x

Genome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3

Indian J Microbiol. 2019 Mar;59(1):109-111. doi: 10.1007/s12088-018-0761-x. Epub 2018 Sep 25.

Authors

Yuriy Kuzhyk¹, Maria Lopatniuk², Andriy Luzhetskyy², Victor Fedorenko¹, Bohdan Ostash¹

Affiliations

¹ 1Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Hrushevskoho St. 4, Rm. 102, Lviv, 79005 Ukraine.
² 2Actinobacteria Metabolic Engineering Group, Helmholtz-Institute for Pharmaceutical Research Saarland, Saarland University, Campus C2 3, 66123 Saarbrücken, Germany.

Abstract

Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by Streptomyces ghanaensis ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene bldA and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.

Keywords: Genome engineering; Integrative vectors; Moenomycins; Streptomyces ghanaensis.