1. Visualization by hand & wrist plain film radiographs: Standard posteroanterior (PA) view of the hand and wrist; Effective radiation received during each exposure is between 0.0001-0.1 mSV. Typically, left-hand radiographs are utilized (as most individuals are right dominant and the left hand has less chance of being deformed)
Techniques:
A. Greulich and Pyle (GP) atlas : Based on "The Radiographic Atlas of Skeletal Development of the Hand and Wrist" by Dr. William Walter Greulich and Dr. Sarah Idell Pyle (1959). Includes reference standards of the left hand and left wrist until 18 years for females and 19 years for males. Advantage: Simpler and faster method; good reliability for Australian and Middle Eastern ethnicity. Disadvantage: Less reliable in Asians.
B. Tanner Whitehouse (TW2) Method : Based on the level of maturity for 20 selected regions of interest (ROI) in specific bones of hand and wrist in each age population. The development level for each ROI is categorized into specific stages, and given a numerical score for each individual bone. A total maturity score is calculated by summing up all these scores, which then correlates with sex-specific bone age. Advantage: More accurate and more reproducible. Disadvantage: Comparatively more complex and more time-consuming.
C. Gilsanz and Ratibin (GR) Atlas : Digital atlas prepared by Vincent Gilsanz and Osman Ratibin (2005). Includes age- and sex-specific artificial images of skeletal maturity after thoroughly analyzing size, shape, morphology, and density of ossification centers in healthy children (spaced at 6-monthly intervals between 2 and 6 years, and yearly intervals between 7 and 17 years). Advantage: More precise and better quality images, as compared with GP atlas. Disadvantage: Shows fairly similar results in previous studies, however, GR atlas has more outliers.
D. Automated skeletal bone age assessment : Digital radiographs of hand and wrist Xray - Undergoes several stages of processing:
Stage 1: Pre-processing: Image normalized to grayscale, background removed and re-oriented
Stage 2: Segmentation: Desired parts of image separated from the background
Stage 3: Analysis: Selected regions of interest analyzed for bone age by TW2 method or GP atlas
Recently developed software computes bone age by both GP and TW2 methods, which has demonstrated validity across different ethnicities.
E. Capito-hamate planimetry [Choi et al. (2018)] : Defined as the measurement of the sum of areas of the capitate and hamate. Can be assessed using plain radiographs.
2. Visualization by ultrasound (US) : Specialized ultrasound device involving two transducers: one transmitted (ultrasonic waves of 750kHz Frequency) and one receiver. Includes 11 cycles directed at the epiphysis of distal radius and ulna and skeletal age is determined, based on demographics of subject and ultrasound results. Disadvantage: Still needs to be evaluated in multiethnic populations with a large sample size.
3. Visualization by Magnetic Resonance Imaging (MRI) : Hand and wrist MRI has been evaluated as an option for bone age estimation (GP and TW2 methods) (Urschler et al. 2016); nevertheless, this technique still needs further validation.
4. Visualization of elbow ossification : Sauvegrain et al. (1962) developed a full 27-point scoring system for bone age assessment including the assessment of four growth centers (lateral condyle and epicondyle, trochlea, olecranon apophysis, and proximal radial epiphysis) on AP and lateral radiographs of the left elbow. This assessment modality involves relatively greater radiation exposure. The order of appearance and fusion of different ossification centers around the elbow and their correlation with chronological age has been described : capitellum (0-1 year; 10-15 years), radial head (2-6 years; 12-16 years), medial epicondyle (2-8 years; 13 years), trochlea (5-11 years; 10-18 years), olecranon (6-11 years; 13-16 years) and lateral epicondyle (8-13 years; 12-16 years). Appearance and fusion for all these ossification centers (except capitellum and radial head) have been demonstrated to be earlier in girls than boys.
5. Visualization of humeral head ossification : Li et al. (2018) had recently described the 5-staged humeral head ossification classification system:
Stage 1 includes an incompletely ossified lateral epiphysis
Stage 2 demonstrates increased ossification of the lateral epiphysis, with a lateral curved margin
Stages 3 to 5 demonstrate collinearity between lateral margin of epiphysis and metaphysis; in stage 3, the lateral half of the physis is open without obvious fusion, in stage 4, the lateral half of physis is partly fused, and in stage 5, the lateral half of physis is completely fused
It has been demonstrated that peak height velocity (PHV) correlates between stages 2 and 3.
6. Visualization of the clavicle : A secondary ossification center develops at the medial end of the clavicle during adolescence and undergoes fusion at 22 years. Best bone to image for bone age assessment between 18 and 22 years of age. Conventional radiographs - not much use to assess; Conventional or Spiral CT - Concerns regarding radiation exposure; and MRI (3-Tesla versus 1.5 Tesla) - Can be a radiation-free option, however, further research to develop specific protocols needed.
7. Visualization of iliac bone :
A. By pelvic radiographs: Risser sign - Based on the ossification of iliac crest apophysis. However, not a reliable or commonly used technique.
B. Non-ionizing Imaging: Ultrasonography as a technique to assess bone age using iliac crest apophysis ossification - has also been considered. However, this technique still requires validation.
8. Visualization of the femoral head : Bone age can be calculated by assessing the depth of epiphyseal cartilage of the femoral head, in contrast to visualizing bony epiphyseal femur. As the ossification process occurs, most of the cartilage undergoes replacement by bone and hyaline articular cartilage. Ultrasound assessment has been studied to evaluate femoral head ossification; however, the evidence is still unequivocal.
9. Modified Oxford Score : Described by Stasikelis et al. assessed bone age on the basis of three consecutive stages of maturation of triradiate cartilage (TRC), femoral epiphysis, greater trochanter, and lesser trochanter. The total score ranges from 16 to 26. There is a solid body of literature regarding the role of this technique in predicting the contralateral slip in patients with SCFE. Modified Oxford score of 16 to 18 corresponds to increased contralateral slips in SCFE and 19 to 21 corresponds to PHV.
10. Calcaneal apophyseal ossification : Nicholson et al. (2016) described a 5-staged bone age assessment based on calcaneal apophysis. Stage 0 - No ossification of the apophysis
Stage 1 - Apophysis covers less than 50% of the metaphysis
Stage 2 - Apophysis covers more than 50% of the metaphysis
Stage 3 - Apophysis has extended fully over the plantar surface and continues to extend over the dorsal surface without evidence of fusion
Stage 4 - Fusion of the apophysis to metaphysis begins
Stage 5 - Fusion is complete
Calcaneal stages 0 to 2 corresponded to high chances of the contralateral slip in SCFE and stage 3 corresponds closely to PHV
11. Visualization of cervical maturation : Schelgl et al. (2017) described the role of EOS 2D/3D imaging in the assessment of bone age, based on cervical vertebral body morphologies (Hassel–Farman Bone Age Stages - initiation, acceleration, transition, deceleration, maturation and completion stages).
12. Visualization of Dental Maturity: Predominantly studies for forensic purposes. Mineralization of teeth is much less affected by nutritional or endocrine disorders than the skeletal system.
Techniques:
A. Atlas method : Based on atlas containing standard age-matched orthopantomographic images, developed by Scour et al. (1944) This was further modified by Moorrees et al. and Anderson et al., where they defined dentition stages of all teeth.
B. Scoring method : Numerical method devised by Demirjian et al. (1973), involving maturity score of each tooth, based on the level of dentition. A total maturity score is calculated by adding individual maturity scores. It has been demonstrated to show good correlation with the GP method of bone age estimation.
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