Abstract
The optical resolution of racemic compounds by stereoselective glucosylation was investigated using plant glucosyltransferase from Phytolacca americana expressed in recombinant Escherichia coli. The glucosyltransferase glucosylated chemoselectively the phenolic hydroxyl group of phenol compounds. The (R)-stereoselective glucosylation of (RS)-denopamine by glucosyltransferase occurred to give (R)-denopamine β-D-glucoside.
MeSH terms
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Escherichia coli / metabolism*
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Ethanolamines / chemistry*
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Plant
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Glucosyltransferases / chemistry
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Glucosyltransferases / metabolism*
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Molecular Structure
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Phytolacca americana / enzymology*
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Phytolacca americana / metabolism
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Plant Proteins / genetics
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Plant Proteins / metabolism
Substances
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Ethanolamines
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Plant Proteins
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Glucosyltransferases
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denopamine