Comprehensive structural characterization of phenolics in litchi pulp using tandem mass spectral molecular networking

Qiang Lyu; Ting-Hao Kuo; Chongde Sun; Kunsong Chen; Cheng-Chih Hsu; Xian Li

doi:10.1016/j.foodchem.2019.01.001

Comprehensive structural characterization of phenolics in litchi pulp using tandem mass spectral molecular networking

Food Chem. 2019 Jun 1:282:9-17. doi: 10.1016/j.foodchem.2019.01.001. Epub 2019 Jan 3.

Authors

Qiang Lyu¹, Ting-Hao Kuo², Chongde Sun³, Kunsong Chen³, Cheng-Chih Hsu⁴, Xian Li⁵

Affiliations

¹ Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China; Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
² Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan.
³ Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China.
⁴ Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan. Electronic address: ccrhsu@ntu.edu.tw.
⁵ Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. Electronic address: xianli@zju.edu.cn.

PMID: 30711110
DOI: 10.1016/j.foodchem.2019.01.001

Abstract

Phenolic compounds are a large class of plant secondary metabolites with various health-promoting effects, and are known for their structural diversity. Therefore, high efficiency characterization of phenolic profiles is of key importance in identifying their potential bioactivity. In the present study, Global Natural Products Social (GNPS) Molecular Networking was applied to trace the phenolic compounds in plants, which allowed the characterization of 9 procyanidins and 11 flavonoid glycosides (di-, tri-, or tetra-saccharides of kaempferol, quercetin, isorhamnetin and myricetin) in litchi pulp extracts. Six compounds were reported for the first time in litchi pulp. In addition, quercetin-3-O-rutinoside-(1 → 2)-O-rhamnoside, the most abundant flavonoid glycoside in litchi pulp, was proved to have considerable α-glucosidase inhibitory activity, illustrating the anti-diabetic potential of phenolic-rich litchi pulp extracts.

Keywords: (–)-Epicatechin (PubChem CID: 72276); GNPS; Isorhamnetin-3-O-rutinoside (PubChem CID: 5481663); Kaempferol-3-O-rutinoside (PubChem CID: 5318767); LC-ESI-MS; Litchi chinensis pulp; Phenolic compounds identification; Procyanidin B2 (PubChem CID: 122738); Quercetin-3-O-rutinoside (PubChem CID: 5280805); α-Glucosidase inhibitory activity.

MeSH terms

Chromatography, High Pressure Liquid
Fruit / chemistry
Fruit / metabolism
Glycosides / analysis
Litchi / chemistry*
Litchi / metabolism
Magnetic Resonance Spectroscopy
Phenols / analysis*
Plant Extracts / chemistry
Proanthocyanidins / analysis
Tandem Mass Spectrometry*

Substances

Glycosides
Phenols
Plant Extracts
Proanthocyanidins