Differential expression of salivary S100A7 in oral submucous fibrosis

Muhammad Arsalan Raffat; Naila Irum Hadi; Mervyn Hosein; Adnan Mustafa Zubairi; Sana Ikram; Zohaib Akram

doi:10.1016/j.sdentj.2018.09.007

Differential expression of salivary S100A7 in oral submucous fibrosis

Saudi Dent J. 2019 Jan;31(1):39-44. doi: 10.1016/j.sdentj.2018.09.007. Epub 2018 Sep 24.

Authors

Muhammad Arsalan Raffat¹, Naila Irum Hadi², Mervyn Hosein³, Adnan Mustafa Zubairi⁴, Sana Ikram⁵, Zohaib Akram⁶

Affiliations

¹ Department of Oral and Maxillofacial Pathology, Ziauddin College of Dentistry, Ziauddin University, 75600 Karachi, Pakistan.
² Department of Pathology, Islamabad Medical & Dental College, 45400 Islamabad, Pakistan.
³ Ziauddin College of Dentistry, Ziauddin University, 75600 Karachi, Pakistan.
⁴ Department of Chemical Pathology, Ziauddin University Hospital, 75600 Karachi, Pakistan.
⁵ Department of Oral Biology, Ziauddin College of Dentistry, Ziauddin University, 75600 Karachi, Pakistan.
⁶ Department of Periodontology, Faculty of Dentistry, Ziauddin University, 75600 Karachi, Pakistan.

Abstract

Aim: To investigate the expression of salivary S100A7 levels among patients with oral submucous fibrosis (OSF) and healthy controls.

Method: A total number of 60 participants were included in the study (30 OSF cases and 30 healthy controls). Demographic data was collected using a structured baseline questionnaire. Salivary S100A7 levels were quantified using enzyme-linked immunosorbent assay. Data was analyzed using Student t-test. Pearson correlation test was used to evaluate correlation between S100A7 levels and independent variables such as frequency and duration of areca nut use, gutka use, and mouth opening.

Results: The mean value of salivary S100A7 for OSF group was 0.275 ng/ml, whereas mean value of salivary S100A7 for healthy controls was 0.195 ng/ml. Student t-test indicated that there was statistically significantly higher levels of S100A7 in OSF group as compared to healthy controls (p < .001). When the clinical variables of individual groups were analysed, a significant negative correlation was found between salivary S100A7 and duration of areca nut (p = .009) and gutka chewing (p = .03), whereas a significant positive correlation was found for mouth opening (p = .04).

Conclusion: OSF presented higher levels of salivary S100A7 levels as compared with healthy individuals and may be used as surrogate measure to identify subjects at risk for OSF.

Keywords: HRP, horseradish peroxidase; NF-κB, nuclear factor kappa-light-chain-enhancer of activated B cells; OSCC, oral squamous cell carcinoma; OSF, oral submucous fibrosis; Oral submucous fibrosis; Psoriasin, S100A7; RAGEs, receptors of advanced glycated end products; ROS, reactive oxygen species; S100A7 protein; SPSS, Statistical Package for the Social Sciences; Saliva; TMB, tetramethylbenzidine; UWS, unstimulated whole saliva.