Functional Analysis of Root microRNAs by a Constitutive Overexpression Approach in a Composite Plant System

Damien Formey; José Ángel Martín-Rodríguez; Georgina Hernández

doi:10.1007/978-1-4939-9042-9_16

Functional Analysis of Root microRNAs by a Constitutive Overexpression Approach in a Composite Plant System

Methods Mol Biol. 2019:1932:215-226. doi: 10.1007/978-1-4939-9042-9_16.

Authors

Damien Formey¹, José Ángel Martín-Rodríguez¹, Georgina Hernández²

Affiliations

¹ Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México (UNAM), Cuernavaca, Morelos, Mexico.
² Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México (UNAM), Cuernavaca, Morelos, Mexico. gina@ccg.unam.mx.

PMID: 30701503
DOI: 10.1007/978-1-4939-9042-9_16

Abstract

Agrobacterium-mediated transformation is a fast and efficient method for genome modification in plants. In this protocol, we apply this technique for the analysis of root microRNA functionality. The induction of hairy roots constitutively overexpressing a given microRNA precursor allows us, in a simple way, to modify the accumulation of specific mature microRNA and analyze the consequence of this alteration on a phenotype of interest. This method generates ready-to-phenotype "composite plants" with untransformed aerial part and microRNA-overexpressing root system, in about 20 days.

Keywords: Agrobacterium rhizogenes; Hairy roots; Overexpression; T-DNA; microRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agrobacterium / genetics*
MicroRNAs / genetics*
Plant Roots / genetics*
Plants, Genetically Modified / genetics
Rhizobium / genetics
Transformation, Genetic / genetics

Substances

MicroRNAs