A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy

Methods Mol Biol. 2019:1932:187-195. doi: 10.1007/978-1-4939-9042-9_14.

Abstract

We present a simple protocol to image floral tissues with confocal laser scanning microscopy (CLSM). Recently, new imaging techniques have emerged that improve the image quality of plant tissues. In this protocol, as an example, we focus on the fluorescence detection of the miRNA MIR164c precursor. Briefly, the method involves tissue clearing, cell wall staining, and the visualization of fluorescence in tissues in young floral buds of Arabidopsis with CLSM with the use of water dipping lenses.

Keywords: ClearSee; Confocal microscopy; Expression; Fluorescence; MIR164; RS2200; Staining; miRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / ultrastructure*
  • Cell Wall / ultrastructure
  • Fluorescence
  • Microscopy, Confocal / methods*
  • RNA Precursors / genetics
  • Staining and Labeling / methods

Substances

  • RNA Precursors