Determination of Auxin Flow During Phototropic Responses Using Fluorescent Auxin Analogs

Tatsuya Sakai

doi:10.1007/978-1-4939-9015-3_12

Determination of Auxin Flow During Phototropic Responses Using Fluorescent Auxin Analogs

Methods Mol Biol. 2019:1924:157-163. doi: 10.1007/978-1-4939-9015-3_12.

Author

Tatsuya Sakai¹

Affiliation

¹ Graduate School of Science and Technology, Niigata University, Niigata, Japan. tsakai@gs.niigata-u.ac.jp.

PMID: 30694473
DOI: 10.1007/978-1-4939-9015-3_12

Abstract

Altered auxin distribution patterns and the formation of a lateral auxin gradient are often discussed in the context of phototropism. The DR5rev::GFP auxin reporter gene is commonly used in phototropism research in Arabidopsis. This study describes a fluorescent auxin analog, NBD-NAA, as an additional tool for the determination of auxin flow during phototropic responses.

Keywords: Arabidopsis; Auxin gradient; Fluorescent auxin analog; NBD-NAA; Phototropism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / metabolism
Arabidopsis / physiology
Arabidopsis Proteins / metabolism
Indoleacetic Acids / metabolism*
Phototropism / physiology*

Substances

Arabidopsis Proteins
Indoleacetic Acids