Study of structural and magnetic properties and heat induction of gadolinium-substituted manganese zinc ferrite nanoparticles for in vitro magnetic fluid hyperthermia

S V Jadhav; P S Shewale; B C Shin; M P Patil; G D Kim; A A Rokade; S S Park; R A Bohara; Y S Yu

doi:10.1016/j.jcis.2019.01.063

Study of structural and magnetic properties and heat induction of gadolinium-substituted manganese zinc ferrite nanoparticles for in vitro magnetic fluid hyperthermia

J Colloid Interface Sci. 2019 Apr 1:541:192-203. doi: 10.1016/j.jcis.2019.01.063. Epub 2019 Jan 17.

Authors

S V Jadhav¹, P S Shewale², B C Shin³, M P Patil⁴, G D Kim⁴, A A Rokade⁵, S S Park⁵, R A Bohara⁶, Y S Yu⁷

Affiliations

¹ Convergence of Smart Sensors Institute, Dong-Eui University, Busan, Republic of Korea.
² Convergence of Smart Sensors Institute, Dong-Eui University, Busan, Republic of Korea; Micro and Nano Engineering Laboratory, Department of Electronic Engineering, Sogang University, Seoul, Republic of Korea.
³ Electronics Ceramics Center, Dong-Eui University, Busan, Republic of Korea.
⁴ Department of Microbiology, College of Natural Sciences, Pukyong National University, Busan, Republic of Korea.
⁵ Department of Industrial Chemistry, College of Engineering, Pukyong National University, Busan, Republic of Korea.
⁶ CURAM, Biomedical Sciences, National University of Ireland Galway, Galway, Ireland; Center for Interdisciplinary Research, D. Y. Patil University, Kolhapur 416006, MS, India.
⁷ Convergence of Smart Sensors Institute, Dong-Eui University, Busan, Republic of Korea; Department of Radiological Science, Dong-Eui University, Busan, Republic of Korea. Electronic address: ysyu@deu.ac.kr.

PMID: 30690263
DOI: 10.1016/j.jcis.2019.01.063

Abstract

This article outlines the synthesis of gadolinium (Gd)-doped manganese zinc ferrite magnetic nanoparticles (MNPs) as potential magnetic carriers for magnetic fluid hyperthermia (MFH). MNPs with high specific loss power (SLP; 146 W/g) have been developed and used for an in vitro hyperthermia study. The treatment of MFH is fruitful if there is an adequate number of MNPs in tumor cells with the highest SLP to rapidly generate heat while minimizing thermal injury to surrounding healthy tissue. X-ray diffraction patterns of the studied particles confirm the formation of a cubic spinel structure. Field emission scanning electron micrographs showed homogeneous distributions of particles with some agglomerates with a granular appearance. Transmission electron microscopy analysis showed the presence of agglomerated spherical particles at the surface. The substitution of Gd resulted in superparamagnetism at room temperature as confirmed by vibrating sample magnetometer analysis. The estimated saturation magnetization reduced from 48.6 to 28.2 emu/g with an increase in Gd concentration. However, the coercivity increased from 1093 Oe to 1597 Oe. Field cooled and zero field cooled measurements showed Curie temperatures from 315 to 326 K, as required for MFH applications. Cell viability measurements indicated that the MNPs are nontoxic to A549 cells for the studied concentrations of particle fraction and a contact time of up to 24 h. The interaction of the MNPs with A549 cells was highlighted from an image captured by an inverted microscope. In order to treat cancer in vivo, an in vitro hyperthermia study has initially been carried out with A549 cells.

Keywords: Cell viability; Co-precipitation; Curie temperature; Hyperthermia; MNPs; SLP; Superparamagnetism.