Raman tracking the activity of urease in saliva for healthcare

Sen Hu; Yun Gao; Yiping Wu; Xiaoyu Guo; Ye Ying; Ying Wen; Haifeng Yang

doi:10.1016/j.bios.2018.12.059

Raman tracking the activity of urease in saliva for healthcare

Biosens Bioelectron. 2019 Mar 15:129:24-28. doi: 10.1016/j.bios.2018.12.059. Epub 2019 Jan 15.

Authors

Sen Hu¹, Yun Gao¹, Yiping Wu², Xiaoyu Guo¹, Ye Ying¹, Ying Wen¹, Haifeng Yang³

Affiliations

¹ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China.
² The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China. Electronic address: yipingwu@shnu.edu.cn.
³ The Education Ministry Key Lab of Resource Chemistry, Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai 200234, China. Electronic address: hfyang@shnu.edu.cn.

PMID: 30682685
DOI: 10.1016/j.bios.2018.12.059

Abstract

The detection of urease activity in the oral cavity is considered to be an efficient way to prevent dental caries and also to screen for helicobacter pylori infection. Herein, a rapid surface enhanced Raman scattering (SERS) method is proposed to determine the activity of urease by using inositol hexaphosphate (IP₆) stabilized silver nanoparticles (AgNPs@IP₆) as an efficient SERS-active substrate. The determination was achieved by monitoring the SERS peak intensity of urea at 1003 cm^-1. With urease increase, the response of urea at 1003 cm^-1 decreases gradually, indicating the two has good correlation. A linear relationship between the absolute value of signal drop and urease concentration is observed in a range from 2.35 to 37.5 μg/mL. In addition, the rapid SERS method was used to evaluate the activity of urease in real sample of saliva without any pretreatment, exhibiting a promising potential for biomedical application.

Keywords: Healthcare; Surface-enhanced Raman scattering; Urea; Urease activity.

Publication types

Evaluation Study

MeSH terms

Dental Caries
Enzyme Assays / methods*
Humans
Limit of Detection
Metal Nanoparticles / chemistry
Phytic Acid / chemistry
Saliva / enzymology*
Saliva / metabolism
Silver / chemistry
Spectrum Analysis, Raman / methods*
Urea / analysis
Urea / metabolism
Urease / analysis*
Urease / metabolism

Substances

Silver
Phytic Acid
Urea
Urease