To achieve the simultaneous removal of NH4+-N and NO3--N in F--containing semiconductor wastewater by coupled S0-driven autotrophic denitrification and Anammox process, the effect of variable F- concentration on the Anammox process was investigated by batch experiments. The denitrifying ammonium oxidation (Deamox) reactor was then started-up to explore the feasibility of the coupling of Anammox and sulfur autotrophic denitrification (SADN) for the treatment of semiconductor wastewater. Short-term variation of F- concentration has an obviously effect on the activity of Anammox sludge, but didn't affect the nitrogen conversion rate. The activity of Anammox obviously decreased after long-term operation of the Deamox reactor when influent F- concentrations reached 552 mg/L. The sensitivity of Anammox bacteria to F- concentration is stronger than that of SADN bacteria. Total nitrogen removal efficiency of 98% and total nitrogen removal rate of 4.11 kg/(m3·d) were achieved in the Deamox reactor, when the F- was pre-treated by calcium ions. Moreover, the high-throughput 16S rRNA gene sequence analysis indicated that variation in F- concentrations could influence the structure and functional of microbial communities in the Deamox process. Candidatus Kuenenia, Thiobacillus and Sulfurimonas were main functional bacteria that achieved symbiotic.
Keywords: Anammox; Coupling; Denitrifying ammonium oxidation (Deamox); Fluoride; Sulfur autotrophic denitrification (SADN).
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