Up-regulation of antioxidative proteins TRX1, TXNL1 and TXNRD1 in the cortex of PTZ kindling seizure model mice

PLoS One. 2019 Jan 24;14(1):e0210670. doi: 10.1371/journal.pone.0210670. eCollection 2019.

Abstract

Oxidative stress has been considered as one of pathogenesis of brain damage led by epilepsy. Reducing oxidative stress can ameliorate brain damage during seizures. However, expression levels of important antioxidative enzymes such as thioredoxin-1 (TRX1), thioredoxin-like 1 protein (TXNL1) and thioredoxin reductase 1 (TXNRD1) during seizures have not been investigated. In this study, we examined protein and mRNA expression levels of TRX1, TXNL1 and TXNRD1 in different brain regions in PTZ induced seizure model mice. We found that protein expression levels of TRX1, TXNL1 and TXNRD1 are simultaneously up-regulated by 2- or 3-fold in the cortex of both acute and chronic seizure model mice. But there is no unified expression pattern change of these enzymes in the hippocampus, cerebellum and diencephalon in the seizure model mice. Less extent up-regulation of mRNA expression of these enzymes were also observed in the cortex of seizure mice. These data suggest that antioxidative enzymes may provide a protective effect against oxidative stress in the cortex during seizures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Disease Models, Animal
  • Kindling, Neurologic / genetics
  • Kindling, Neurologic / metabolism*
  • Male
  • Mice
  • Seizures / genetics
  • Seizures / metabolism*
  • Thioredoxin Reductase 1 / genetics
  • Thioredoxin Reductase 1 / metabolism*
  • Thioredoxins / genetics
  • Thioredoxins / metabolism*

Substances

  • Txnl1 protein, mouse
  • Thioredoxins
  • Thioredoxin Reductase 1
  • Txnrd1 protein, mouse

Grants and funding

This work was supported by Natural Science Foundation of China (NSFC) grant to Z.Z. (81471314, 81671090), Natural Science Foundation of Jiangsu Province to Z.Z (BK20151170). Xuzhou Science and Technology Program (16631635) to Z.Z. NSFC grant to Q.-Y.T. (31671212), Xuzhou Science and Technology Program to Q.-Y.T (KC16H0230). The General Project of Natural Science in College and University in Jiangsu Province to J.T Yu (14KJB180023). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.