Abstract
Calcium ion (Ca2+) is a ubiquitous intracellular messenger able to generate versatile intracellular signals that modulate a large variety of functions in virtually every cell type. Chemical and genetic biosensors, targeted to different subcellular compartments, have been developed and continuously improved to monitor Ca2+ dynamics in living cells. Here we describe the usage of Förster resonance energy transfer (FRET)-based Cameleon probes to investigate Ca2+ influx across the plasma membrane (PM) or Ca2+ release from the main intracellular Ca2+ store, the endoplasmic reticulum (ER).
Keywords:
Ca2+ imaging; Ca2+ signaling; Cameleon; Endoplasmic reticulum; FRET; IP3 receptor; SOCE.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry
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Bacterial Proteins / metabolism*
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Calcium / analysis
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Calcium / metabolism*
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Calcium Signaling
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Cations, Divalent / analysis
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Cations, Divalent / metabolism
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Cell Line
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Cell Membrane / metabolism*
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Endoplasmic Reticulum / metabolism*
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Fluorescence Resonance Energy Transfer / methods*
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / metabolism*
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Green Fluorescent Proteins / chemistry
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Green Fluorescent Proteins / metabolism*
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HeLa Cells
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Humans
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Luminescent Proteins / chemistry
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Luminescent Proteins / metabolism*
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Optical Imaging / methods
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Peptides / chemistry
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Peptides / metabolism
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Protein Binding
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Protein Conformation
Substances
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Bacterial Proteins
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Cations, Divalent
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Fluorescent Dyes
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Luminescent Proteins
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Peptides
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yellow fluorescent protein, Bacteria
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Green Fluorescent Proteins
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Calcium