Minimal Resection Takes Place during Break-Induced Replication Repair of Collapsed Replication Forks and Is Controlled by Strand Invasion

Cell Rep. 2019 Jan 22;26(4):836-844.e3. doi: 10.1016/j.celrep.2018.12.108.

Abstract

A natural and frequently occurring replication problem is generated by the action of topoisomerase I (Top1). Trapping of Top1 in a cleavage complex on the DNA generates a protein-linked DNA nick (PDN), which upon DNA replication can be transformed into a one-ended double-strand break (DSB). Break-induced replication (BIR) has been recognized as a critical repair mechanism of one-ended DSBs. Here, we have investigated resection at a one-ended DSB formed exclusively during replication due to Top1-mimicking damage. We show that resection is minimal, and only when strand invasion is abolished is extensive resection detected. When DNA synthesis is slowed by hydroxyurea treatment, extended resection is not observed, which suggests that strand invasion and/or heteroduplex formation restrains resection. Our results demonstrate that the BIR pathway acting during S phase is tailored to prevent hazardous effects of naturally and frequently occurring DNA breaks such as Top1-generated PDNs.

Keywords: break-induced replication; double-strand break repair; resection; topoisomerase-generated damage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Breaks, Double-Stranded*
  • DNA Breaks, Single-Stranded*
  • DNA Replication*
  • DNA Topoisomerases, Type I* / genetics
  • DNA Topoisomerases, Type I* / metabolism
  • DNA, Fungal* / biosynthesis
  • DNA, Fungal* / genetics
  • Hydroxyurea / pharmacology
  • Saccharomyces cerevisiae Proteins* / genetics
  • Saccharomyces cerevisiae Proteins* / metabolism
  • Saccharomyces cerevisiae* / genetics
  • Saccharomyces cerevisiae* / metabolism

Substances

  • DNA, Fungal
  • Saccharomyces cerevisiae Proteins
  • TOP1 protein, S cerevisiae
  • DNA Topoisomerases, Type I
  • Hydroxyurea