CRISPR-Cas: a tool for cancer research and therapeutics

Hao Yin; Wen Xue; Daniel G Anderson

doi:10.1038/s41571-019-0166-8

CRISPR-Cas: a tool for cancer research and therapeutics

Nat Rev Clin Oncol. 2019 May;16(5):281-295. doi: 10.1038/s41571-019-0166-8.

Authors

Hao Yin^{1

2}, Wen Xue^{3

4

5}, Daniel G Anderson^{6

7

8

9}

Affiliations

¹ Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China. haoyin@whu.edu.cn.
² Medical Research Institute, Wuhan University, Wuhan, China. haoyin@whu.edu.cn.
³ RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, MA, USA. wen.xue@umassmed.edu.
⁴ Department of Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA, USA. wen.xue@umassmed.edu.
⁵ Department of Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA. wen.xue@umassmed.edu.
⁶ David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology (MIT), Cambridge, MA, USA. dgander@mit.edu.
⁷ Department of Chemical Engineering, MIT, Cambridge, MA, USA. dgander@mit.edu.
⁸ Harvard-MIT Division of Health Sciences & Technology, Cambridge, MA, USA. dgander@mit.edu.
⁹ Institute of Medical Engineering and Science, MIT, Cambridge, MA, USA. dgander@mit.edu.

PMID: 30664678
DOI: 10.1038/s41571-019-0166-8

Abstract

In the past decade, the development of a genome-editing technology mediated by CRISPR has made genetic engineering easier than ever, both in vitro and in vivo. CRISPR systems have enabled important advances in cancer research by accelerating the development of study models or as a tool in genetic screening studies, including those aiming to discover and validate therapeutic targets. In this Review, we discuss these applications as well as new potential uses of CRISPR to assist in cancer detection or the development of anticancer therapies.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
CRISPR-Cas Systems
Gene Editing / methods*
Genetic Vectors / administration & dosage
Humans
Mice
Neoplasms / diagnosis*
Neoplasms / genetics
Neoplasms / therapy*
Xenograft Model Antitumor Assays