Single particle ICP-MS-based absolute and relative quantification of E. coli O157 16S rRNA using sandwich hybridization capture

Analyst. 2019 Feb 25;144(5):1725-1730. doi: 10.1039/c8an02063a.

Abstract

Herein, a novel 16S rRNA detection platform was achieved by combining a sandwich hybridization reaction, a single-molecule magnetic capture, and single particle-inductively coupled plasma mass spectrometry amplification. The assay was developed for the direct detection of RNA from dangerous human pathogens and enabled absolute and high-precision quantification of a target with a detection limit of 10 fM.

MeSH terms

  • Animals
  • Base Sequence
  • Biological Assay / methods*
  • DNA Primers / genetics*
  • DNA Primers / radiation effects
  • Escherichia coli O157 / genetics
  • Food Contamination / analysis
  • Gold / chemistry
  • Light
  • Limit of Detection
  • Magnetic Phenomena
  • Mass Spectrometry / methods*
  • Metal Nanoparticles / chemistry
  • Milk / microbiology
  • Nucleic Acid Hybridization
  • Photochemistry / methods
  • RNA, Ribosomal, 16S / analysis*
  • RNA, Ribosomal, 16S / genetics*

Substances

  • DNA Primers
  • RNA, Ribosomal, 16S
  • Gold