Abstract
Herein, a novel 16S rRNA detection platform was achieved by combining a sandwich hybridization reaction, a single-molecule magnetic capture, and single particle-inductively coupled plasma mass spectrometry amplification. The assay was developed for the direct detection of RNA from dangerous human pathogens and enabled absolute and high-precision quantification of a target with a detection limit of 10 fM.
MeSH terms
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Animals
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Base Sequence
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Biological Assay / methods*
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DNA Primers / genetics*
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DNA Primers / radiation effects
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Escherichia coli O157 / genetics
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Food Contamination / analysis
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Gold / chemistry
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Light
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Limit of Detection
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Magnetic Phenomena
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Mass Spectrometry / methods*
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Metal Nanoparticles / chemistry
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Milk / microbiology
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Nucleic Acid Hybridization
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Photochemistry / methods
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RNA, Ribosomal, 16S / analysis*
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RNA, Ribosomal, 16S / genetics*
Substances
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DNA Primers
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RNA, Ribosomal, 16S
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Gold