Screening and enrichment of high lipid producing microalgal consortia

Jyoti Sharma; Smita S Kumar; Narsi R Bishnoi; Arivalagan Pugazhendhi

doi:10.1016/j.jphotobiol.2019.01.002

Screening and enrichment of high lipid producing microalgal consortia

J Photochem Photobiol B. 2019 Mar:192:8-12. doi: 10.1016/j.jphotobiol.2019.01.002. Epub 2019 Jan 9.

Authors

Jyoti Sharma¹, Smita S Kumar², Narsi R Bishnoi¹, Arivalagan Pugazhendhi³

Affiliations

¹ Department of Environmental Science & Engineering, Guru Jambheshwar University of Science & Technology, Hisar 125001, Haryana, India.
² Centre for Rural Development & Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
³ Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam. Electronic address: arivalagan.pugazhendhi@tdtu.edu.vn.

PMID: 30660068
DOI: 10.1016/j.jphotobiol.2019.01.002

Abstract

Lipid content is the main parameter for the evaluation of microalgae towards biodiesel production. The present study was aimed at the screening of desirable algal consortia based on lipid content for further processing into biofuel. Twenty different algal consortia were investigated for lipid content qualitatively by Nile Red and quantitatively by gravimetric method and FTIR. Nile Red is used as a fluorophore for physically characterizing the lipid body in algal cultures. The yellow fluorescence in all the samples confirmed the presence of lipid content when observed under a fluorescent microscope. Lipid contents of algal consortia estimated by gravimetry varied from 3.53-16.21%, which was higher than that assessed by FTIR analysis. FTIR spectroscopy can be used for the quantitative estimation of lipid content, protein and carbohydrate concentration. FTIR analysis of the samples revealed the presence of lipid content by virtue of methylene and methyl stretching peaks at 2800-3000 cm^-1. The appearance of stretching vibrations at 1600-1700 cm^-1 and 1000-1200 cm ^-1 obtained by FTIR spectra confirmed the presence of protein contents and carbohydrate, respectively in all the samples. Two algal consortia SM 4 and SM13 were screened out on the basis of high lipid concentration. These algal samples can be further optimized for enhanced biofuel production.

Keywords: Consortium; FTIR; Gravimetry; Lipid; Microalgae; Nile Red.

MeSH terms

Biofuels*
High-Throughput Screening Assays
Lipid Metabolism*
Lipids / analysis*
Microalgae / chemistry*
Spectroscopy, Fourier Transform Infrared

Substances

Biofuels
Lipids