Developing cysteamine-modified SERS substrate for detection of acidic pigment with weak surface affinity

Huan He; Pan Li; Xianghu Tang; Dongyue Lin; Anjian Xie; Yuhua Shen; Liangbao Yang

doi:10.1016/j.saa.2019.01.007

Developing cysteamine-modified SERS substrate for detection of acidic pigment with weak surface affinity

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Apr 5:212:293-299. doi: 10.1016/j.saa.2019.01.007. Epub 2019 Jan 3.

Authors

Huan He¹, Pan Li², Xianghu Tang², Dongyue Lin², Anjian Xie¹, Yuhua Shen³, Liangbao Yang⁴

Affiliations

¹ School of Chemistry and Chemical Engineering, Anhui University, Hefei 230601, China.
² Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei 230031, China.
³ School of Chemistry and Chemical Engineering, Anhui University, Hefei 230601, China. Electronic address: s_yuhua@163.com.
⁴ Institute of Intelligent Machines, Chinese Academy of Sciences, Hefei 230031, China. Electronic address: lbyang@iim.ac.cn.

PMID: 30660061
DOI: 10.1016/j.saa.2019.01.007

Abstract

In this paper, we developed cysteamine-modified surface-enhanced Raman scattering (SERS) substrate for detecting detect trace amount of acidic pigment that shows weak affinity with gold nanoparticles (Au NPs). To realize sensitive and reproducible detection of pigment with weak affinity, the SERS substrate was prepared by attaching cysteamine (CA) to the Au NPs, the acidic pigment molecule could rapidly reached to the surface of Au NPs because of the formation of multi‑hydrogen-bond and electrostatic interaction between the pigment and CA molecule. The proposed method allowed us to detect five kinds of acidic pigment with a limit of 1.0 ppm, which is below the strictest safety limit. Compared with the previous methods, the advantages of the present substrate were its simple substrate preparation, high reproducibility and good universality. Furthermore, the reliable and enough accurate results had been obtained by using of the proposed substrates in the assay of trace pigment in real samples.

Keywords: Acidic pigment; Cysteamine-modified; Hydrogen bond interaction; Surface-enhanced Raman scattering; Weak affinity.