Predominance of blaCTX-M-65 and blaCTX-M-55 in extended-spectrum β-lactamase-producing Escherichia coli from raw retail chicken in South Korea

Hyeeun Park; Jinshil Kim; Sangryeol Ryu; Byeonghwa Jeon

doi:10.1016/j.jgar.2019.01.005

Predominance of bla_CTX-M-65 and bla_CTX-M-55 in extended-spectrum β-lactamase-producing Escherichia coli from raw retail chicken in South Korea

J Glob Antimicrob Resist. 2019 Jun:17:216-220. doi: 10.1016/j.jgar.2019.01.005. Epub 2019 Jan 15.

Authors

Hyeeun Park¹, Jinshil Kim¹, Sangryeol Ryu², Byeonghwa Jeon³

Affiliations

¹ Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 08826, South Korea.
² Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 08826, South Korea. Electronic address: sangryu@snu.ac.kr.
³ Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 08826, South Korea; School of Public Health, University of Alberta, Edmonton, Alberta, Canada. Electronic address: bjeon@snu.ac.kr.

PMID: 30658198
DOI: 10.1016/j.jgar.2019.01.005

Abstract

Objectives: Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) are a serious public health concern worldwide. The aim of this study was to characterise ESBL-EC isolated from raw retail chicken in South Korea.

Methods: The antimicrobial resistance, phylogenetic group and virulence gene prevalence of 67 ESBL-EC isolated from retail chicken in South Korea were investigated.

Results: All of the isolates possessed bla_CTX-M genes, predominantly bla_CTX-M-65 (52.2%) and bla_CTX-M-55 (25.4%), and three isolates harboured both bla_CTX-M-65 and bla_CTX-M-55. More than one-half of the ESBL-EC strains also carried bla_TEM. Antimicrobial susceptibility testing revealed that 98.5% of the strains were multidrug-resistant (MDR). Phylogenetic analysis showed that group A was predominant (56.7%), followed by B1 (19.4%), E (8.9%), B2 (6.0%) and D (6.0%). Virulence genes associated with extraintestinal pathogenic E. coli (ExPEC) were frequently detected in isolates of phylogenetic groups B1, B2, D and E.

Conclusion: The results in this study demonstrate that retail chicken in South Korea is highly contaminated with MDR ESBL-EC and may serve as a reservoir for transmitting ExPEC to humans.

Keywords: Antimicrobial resistance; CTX-M; Chicken; ESBL; Escherichia coli; Extended-spectrum β-lactamase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Chickens / microbiology*
Drug Resistance, Multiple, Bacterial / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Infections / epidemiology
Escherichia coli Proteins / genetics*
Genes, Bacterial / genetics
Humans
Microbial Sensitivity Tests
Phylogeny
Prevalence
Republic of Korea
Virulence / genetics
beta-Lactamases / classification
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
beta-Lactamases
beta-lactamase CTX-M-55, E coli