Immunohistochemical expression of osteonectin, matrix metalloproteinases-9 and Ki-67 in ameloblastoma

K Indirapriyadarsini; Vandana Raghunath; B Venu Naidu; Botu Badari Ramakrishna; Archana Tangudu; K V Lokesh

doi:10.4103/jomfp.JOMFP_271_17

Immunohistochemical expression of osteonectin, matrix metalloproteinases-9 and Ki-67 in ameloblastoma

J Oral Maxillofac Pathol. 2018 Sep-Dec;22(3):446. doi: 10.4103/jomfp.JOMFP_271_17.

Authors

K Indirapriyadarsini¹, Vandana Raghunath², B Venu Naidu³, Botu Badari Ramakrishna⁴, Archana Tangudu⁵, K V Lokesh⁴

Affiliations

¹ Department of Oral and Maxillofacial Pathology and Microbiology, CKS Theja Institute of Dental Science and Research, Tirupati, Andhra Pradesh, India.
² Department of Oral and Maxillofacial Pathology and Microbiology, Narayana Dental College, Nellore, Andhra Pradesh, India.
³ Department of Oral and Maxillofacial Pathology and Microbiology, Anil Neerukonda Institute of Dental Sciences, Visakhapatnam, Andhra Pradesh, India.
⁴ Department of Oral Medicine and Radiology, Anil Neerukonda Institute of Dental Sciences, Visakhapatnam, Andhra Pradesh, India.
⁵ Department of Periodontics, Anil Neerukonda Institute of Dental Sciences, Visakhapatnam, Andhra Pradesh, India.

Abstract

Background: Ameloblastoma (AM) is a benign odontogenic neoplasm characterized by local invasiveness and recurrence. We have evaluated the immunohistochemical expressions of osteonectin (ON), matrix metalloproteinase-9 (MMP-9) and Ki-67 in AM to understand the biologic behavior of this neoplasm.

Aim: The aim of this study is to evaluate the expression of ON, MMP-9 and Ki-67 in AM.

Materials and methods: The study sample included archival tissues embedded in paraffin blocks histopathologically diagnosed as AM (20 cases). Three serial sections of each tissue specimen were stained separately with ON, MMP-9 and Ki-67 (immunohistochemistry stain) and evaluated. The data were analyzed using Spearman rank correlation test.

Results: All AM cases 20/20 (100%) exhibited positive immunostaining for ON, MMP-9 and Ki-67.

Conclusion: Increased expression of ON, MMP-9 and Ki-67 in AM indicates that these molecules might play a role in the regulation and aggressive nature of this neoplasm.

Keywords: Ameloblastoma; Ki-67; matrix metalloproteinase-9; osteonectin.