Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks

Ario Betha Juanssilfero; Prihardi Kahar; Rezky Lastinov Amza; Yopi; Kumar Sudesh; Chiaki Ogino; Bambang Prasetya; Akihiko Kondo

doi:10.1016/j.jbiosc.2018.12.002

Lipid production by Lipomyces starkeyi using sap squeezed from felled old oil palm trunks

J Biosci Bioeng. 2019 Jun;127(6):726-731. doi: 10.1016/j.jbiosc.2018.12.002. Epub 2019 Jan 12.

Authors

Ario Betha Juanssilfero¹, Prihardi Kahar², Rezky Lastinov Amza², Yopi³, Kumar Sudesh⁴, Chiaki Ogino⁵, Bambang Prasetya⁶, Akihiko Kondo⁷

Affiliations

¹ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km 46, 16911 Cibinong, Bogor, West Java, Indonesia.
² Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
³ Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km 46, 16911 Cibinong, Bogor, West Java, Indonesia.
⁴ Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia.
⁵ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan. Electronic address: ochiaki@port.kobe-u.ac.jp.
⁶ National Standardization Agency of Indonesia (BSN), Gedung Badan Pengkajian dan Penerapan Teknologi (BPPT), Jl. M.H. Thamrin No. 8, Jakarta 10340, Indonesia.
⁷ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan; Graduate School of Science, Technology and Innovation (STIN), Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.

PMID: 30642786
DOI: 10.1016/j.jbiosc.2018.12.002

Abstract

The ability of oleaginous yeast Lipomyces starkeyi to efficiently produce lipids when cultivated on sap extracted from felled oil palm trunk (OPT) as a novel inexpensive renewable carbon source was evaluated. OPT sap was found to contain approximately 98 g/L glucose and 32 g/L fructose. Batch fermentations were performed using three different OPT sap medium conditions: regular sap, enriched sap, and enriched sap at pH 5.0. Under all sap medium conditions, the cell biomass and lipid production achieved were approximately 30 g/L and 60% (w/w), respectively. L. starkeyi tolerated acidified medium (initial pH ≈ 3) and produced considerable amounts of ethanol as well as xylitol as by-products. The fatty acid profile of L. starkeyi was remarkably similar to that of palm oil, one of the most common vegetable oil feedstock used in biodiesel production with oleic acid as the major fatty acid followed by palmitic, stearic and linoleic acids.

Keywords: Lipomyces starkeyi; Oil palm trunk; Oleaginous yeast; Sap; Single cell-oil.

MeSH terms

Biomass*
Fermentation
Hydrogen-Ion Concentration
Lipids / biosynthesis*
Lipomyces / metabolism*
Magnoliopsida / chemistry*

Substances

Lipids