Addition of phosphates and chlorhexidine to resin-modified MTA materials

J Biomed Mater Res B Appl Biomater. 2019 Aug;107(6):2195-2201. doi: 10.1002/jbm.b.34315. Epub 2019 Jan 14.

Abstract

To evaluate the properties of experimental mineral trioxide aggregate (MTA) resin-modified materials for root-end filling procedures, varying their compositions regarding the addition of hydroxiapatite (HA) or dicalcium phosphate dihydrate, with or without chlorhexidine digluconate. White MTA (Angelus, Londrina, Brazil) was used as a reference material. Degree of conversion (DC) was evaluated by Fourier transformed infrared (FTIr) spectroscopy (n = 5). Flowability (n = 3) and radiopacity (n = 3) were evaluated following ISO 6876:2001 methods. For splitting tensile strength analysis, cylindrical samples (n = 10) were subjected to compressive load using a universal testing machine (Instron Corporation, Norwood, MA). Water sorption and solubility tests were performed according to ISO 4049:2009 methods. Calcium ion release and pH analysis (n = 10) were evaluated using a pH meter (Orion, Watsonville, CA). Cytotoxicity (n = 8) of materials extracts was evaluated as cell viability percentage. Statistical analysis was performed using Kolmogorov-Smirnov for normal distribution and data was subjected to one-way ANOVA and Tukey test (α = 0.05). Addition of chlorhexidine digluconate reduced DC mean values for experimental materials (<50%). White MTA demonstrated lower flowability (5.3 mm) and higher radiopacity (9.8 mm Al), splitting tensile strength (9.1 MPa), solubility (8.2 μg/mm3 ), calcium ion release (~26.5 ppm), cytotoxicity (55.2%), and pH mean values (10.8), when compared to experimental materials. All groups demonstrated a decrease in calcium release (<85%) and pH (<13%). Formulation containing HA demonstrated similar pH values after 28 days when compared to white MTA. Evaluated experimental resin-modified MTA based materials without chlorhexidine digluconate showed satisfactory results for all physico-chemical properties tested and cytotoxicity. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2195-2201, 2019.

Keywords: endodontics; hydroxyapatite; methacrylates; mineralized tissue/development; regeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aluminum Compounds* / chemistry
  • Aluminum Compounds* / pharmacokinetics
  • Aluminum Compounds* / pharmacology
  • Animals
  • Calcium Compounds* / chemistry
  • Calcium Compounds* / pharmacokinetics
  • Calcium Compounds* / pharmacology
  • Cell Line
  • Chlorhexidine / analogs & derivatives*
  • Chlorhexidine / chemistry
  • Chlorhexidine / pharmacokinetics
  • Chlorhexidine / pharmacology
  • Drug Combinations
  • Fibroblasts / metabolism*
  • Materials Testing*
  • Mice
  • Oxides* / chemistry
  • Oxides* / pharmacokinetics
  • Oxides* / pharmacology
  • Root Canal Filling Materials* / chemistry
  • Root Canal Filling Materials* / pharmacokinetics
  • Root Canal Filling Materials* / pharmacology
  • Silicates* / chemistry
  • Silicates* / pharmacokinetics
  • Silicates* / pharmacology

Substances

  • Aluminum Compounds
  • Calcium Compounds
  • Drug Combinations
  • Oxides
  • Root Canal Filling Materials
  • Silicates
  • mineral trioxide aggregate
  • chlorhexidine gluconate
  • Chlorhexidine