Abstract
Unprecedented 3D nanobiosystems composed of recombinant CotA laccases and citrate-stabilised gold nanoparticles have been successfully achieved by an electrostatic self-assembly strategy. The bioelectrochemical reduction of O2 driven by CotA laccase at the spore coat was mimicked. Consequently key insights into its bioelectrocatalytic function were unravelled.
MeSH terms
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Bacillus subtilis / enzymology
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Biocatalysis
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Dynamic Light Scattering
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Electrochemical Techniques
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Enzymes, Immobilized / chemistry
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Enzymes, Immobilized / metabolism
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Gold / chemistry
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Laccase / genetics
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Laccase / metabolism*
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Metal Nanoparticles / chemistry
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Particle Size
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Spectrophotometry
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Static Electricity
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Surface Plasmon Resonance
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Temperature
Substances
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Bacterial Proteins
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Enzymes, Immobilized
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Recombinant Proteins
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Gold
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Laccase